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Recombinant BCG Expressing ESX-1 of Mycobacterium marinum Combines Low Virulence with Cytosolic Immune Signaling and Improved TB Protection

Authors
 Matthias I. Gröschel  ;  Fadel Sayes  ;  Sung Jae Shin  ;  Wafa Frigui  ;  Alexandre Pawlik  ;  Mickael Orgeur  ;  Robin Canetti  ;  Nadine Honoré  ;  Roxane Simeone  ;  Tjip S. van der Werf  ;  Wilbert Bitter  ;  Sang-Nae Cho  ;  Laleh Majlessi  ;  Roland Brosch 
Citation
 Cell Reports, Vol.18(11) : 2752-2765, 2017 
Journal Title
 Cell Reports 
Issue Date
2017
MeSH
Animals ; BCG Vaccine/immunology* ; Bacterial Proteins/metabolism* ; Cytosol/immunology* ; Genetic Complementation Test ; Host-Pathogen Interactions/immunology ; Immunity, Innate ; Immunization ; Mice, SCID ; Mycobacterium marinum/pathogenicity* ; Phagosomes/metabolism ; Signal Transduction* ; Th1 Cells/immunology ; Tuberculosis/immunology* ; Tuberculosis/microbiology ; Tuberculosis/prevention & control* ; Vaccines, Synthetic/immunology* ; Virulence
Keywords
ESX/type VII secretion ; Mycobacterium marinum ; Mycobacterium tuberculosis ; cytosolic pattern recognition ; innate immune signaling ; recombinant BCG ; tuberculosis ; vaccination
Abstract
Recent insights into the mechanisms by which Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, is recognized by cytosolic nucleotide sensors have opened new avenues for rational vaccine design. The only licensed anti-tuberculosis vaccine, Mycobacterium bovis BCG, provides limited protection. A feature of BCG is the partial deletion of the ESX-1 type VII secretion system, which governs phagosomal rupture and cytosolic pattern recognition, key intracellular phenotypes linked to increased immune signaling. Here, by heterologously expressing the esx-1 region of Mycobacterium marinum in BCG, we engineered a low-virulence, ESX-1-proficient, recombinant BCG (BCG::ESX-1Mmar) that induces the cGas/STING/TBK1/IRF-3/type I interferon axis and enhances AIM2 and NLRP3 inflammasome activity, resulting in both higher proportions of CD8+ T cell effectors against mycobacterial antigens shared with BCG and polyfunctional CD4+ Th1 cells specific to ESX-1 antigens. Importantly, independent mouse vaccination models show that BCG::ESX-1Mmar confers superior protection relative to parental BCG against challenges with highly virulent M. tuberculosis.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/160853
DOI
10.1016/j.celrep.2017.02.057
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
Yonsei Authors
신성재(Shin, Sung Jae) ; 조상래(Cho, Sang Nae)
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