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Current Strategies and Challenges for Purification of Cardiomyocytes Derived from Human Pluripotent Stem Cells

Authors
 Kiwon Ban  ;  Seongho Bae  ;  Young-sup Yoon 
Citation
 Theranostics, Vol.7(7) : 2067-2077, 2017 
Journal Title
 Theranostics 
Issue Date
2017
MeSH
Cell Differentiation ; Cell Separation/methods* ; Humans ; Myocytes, Cardiac/physiology* ; Pluripotent Stem Cells/physiology*
Keywords
Cardiomyocytes ; hPSCs
Abstract
Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) are considered a most promising option for cell-based cardiac repair. Hence, various protocols have been developed for differentiating hPSCs into CMs. Despite remarkable improvement in the generation of hPSC-CMs, without purification, these protocols can only generate mixed cell populations including undifferentiated hPSCs or non-CMs, which may elicit adverse outcomes. Therefore, one of the major challenges for clinical use of hPSC-CMs is the development of efficient isolation techniques that allow enrichment of hPSC-CMs. In this review, we will discuss diverse strategies that have been developed to enrich hPSC-CMs. We will describe major characteristics of individual hPSC-CM purification methods including their scientific principles, advantages, limitations, and needed improvements. Development of a comprehensive system which can enrich hPSC-CMs will be ultimately useful for cell therapy for diseased hearts, human cardiac disease modeling, cardiac toxicity screening, and cardiac tissue engineering.
Files in This Item:
T201702679.pdf Download
DOI
10.7150/thno.19427
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Yoon, Young Sup(윤영섭)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160561
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