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Phosphorylation of Serine 148 in Giardia lamblia End-binding 1 Protein is Important for Cell Division

Authors
 Juri Kim  ;  Hye‐Yeon Lee  ;  Kyu‐Ho Lee  ;  Soon‐Jung Park 
Citation
 JOURNAL OF EUKARYOTIC MICROBIOLOGY, Vol.64(4) : 464-480, 2017 
Journal Title
 JOURNAL OF EUKARYOTIC MICROBIOLOGY 
ISSN
 1066-5234 
Issue Date
2017
MeSH
Aurora Kinases/metabolism* ; Cytokinesis/drug effects ; Cytoskeletal Proteins/genetics ; Cytoskeletal Proteins/metabolism* ; Gene Expression Regulation/drug effects ; Giardia lamblia/drug effects ; Giardia lamblia/metabolism ; Giardia lamblia/physiology* ; Mutagenesis, Site-Directed ; Nuclear Envelope/metabolism ; Phosphorylation ; Protein Kinase Inhibitors/pharmacology ; Protozoan Proteins/genetics ; Protozoan Proteins/metabolism ; Serine/metabolism*
Keywords
Aurora kinase ; cell cycle ; cytokinesis ; division defect ; mutagenesis
Abstract
Giardia lamblia is a unicellular organism, showing a polarity with two nuclei and cytoskeletal structures. Accurate positioning of these organelles is essential for division of G. lamblia, which is poorly understood. Giardia lamblia end-binding 1 (GlEB1) protein and G. lamblia aurora kinase (GlAK) have been shown to modulate microtubule (MT) distribution during cytokinesis. A direct association between GlEB1 and GlAK was demonstrated. Like GlEB1, GlAK was also found at nuclear envelopes and median bodies of G. lamblia. In vitro kinase assays using Giardia lysates immunoprecipitated with anti-GlAK antibodies or recombinant GlAK suggested that GlEB1 is a substrate of GlAK. Site-directed mutagenesis indicated that threonine-205 in GlAK was auto-phosphorylated and that GlAK phosphorylated serine (Ser)-148 in GlEB1. Ectopic expression of a mutant GlEB1 (with conversion of Ser-148 into alanine of GlEB1) resulted in an increased number of Giardia cells with division defects. Treatment of G. lamblia with an AK inhibitor triggered cytokinesis defects, and ectopic expression of a phospho-mimetic mutant GlEB1 (with conversion of Ser-148 into aspartate) rescued the defects in Giardia cell division caused by the AK inhibitor. These results suggested that phosphorylation of GlEB1 played a role in cytokinesis in G. lamblia.
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12384
DOI
10.1111/jeu.12384
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Lee, Hye Yeon(이혜연)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160520
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