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Lysyl oxidase-like 2 is expressed in kidney tissue and is associated with the progression of tubulointerstitial fibrosis

Authors
 SUNG‑EUN CHOI  ;  NARA JEON  ;  HOON YOUNG CHOI  ;  JAE IL SHIN  ;  HYEON JOO JEONG  ;  BEOM JIN LIM 
Citation
 Molecular Medicine Reports, Vol.16(3) : 2477-2482, 2017 
Journal Title
 Molecular Medicine Reports 
ISSN
 1791-2997 
Issue Date
2017
MeSH
Amino Acid Oxidoreductases/analysis ; Amino Acid Oxidoreductases/genetics ; Animals ; Cell Line ; Disease Progression ; Fibrosis ; Gene Expression Regulation ; Humans ; Kidney/metabolism ; Kidney/pathology ; Kidney Glomerulus/metabolism ; Kidney Glomerulus/pathology ; Kidney Tubules/metabolism ; Kidney Tubules/pathology ; Male ; Mice ; Podocytes/metabolism ; Podocytes/pathology ; Renal Insufficiency, Chronic/genetics ; Renal Insufficiency, Chronic/pathology
Abstract
Tubulointerstitial fibrosis is a common end point of chronic kidney diseases, and preventing its progression is key to avoiding renal failure. Transforming growth factor‑β (TGF‑β) and associated molecules promote tubulointerstitial fibrosis; however, effective therapies targeting these molecules have yet to be developed. Lysyl oxidase‑like 2 (LOXL2), which is involved in invasive growth and metastasis of malignant neoplasms, has recently been reported to serve a key role in hepatic and pulmonary fibrosis. However, little is currently known regarding LOXL2 expression in the kidney and its involvement in tubulointerstitial fibrosis. The present study evaluated LOXL2 expression in human and mouse kidney tissues, as well as in cultured renal cells. LOXL2 protein expression was detected in glomerular capillary loops and tubular epithelial cells in human and mouse kidneys. Glomerular LOXL2 was localized to the cytoplasm of podocytes, as determined by double immunofluorescence microscopy using a podocyte marker (synaptopodin). This result was supported by western blot analysis, which demonstrated that LOXL2 protein expression is present in cultured human podocytes and HK‑2 human proximal tubular cells. In addition, the mRNA and protein expression levels of LOXL2 were higher in a mouse model of tubulointerstitial fibrosis compared with in control mice. In addition, immunohistochemistry results demonstrated that LOXL2 is present in the fibrous interstitium and infiltrating mononuclear cells in a mouse model of tubulointerstitial fibrosis. The present study demonstrated that LOXL2 is expressed in compartments of renal tissue, where it appears to contribute to the progression of tubulointerstitial fibrosis.
Files in This Item:
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DOI
10.3892/mmr.2017.6918
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
신재일(Shin, Jae Il) ORCID logo https://orcid.org/0000-0003-2326-1820
임범진(Lim, Beom Jin) ORCID logo https://orcid.org/0000-0003-2856-0133
정현주(Jeong, Hyeon Joo) ORCID logo https://orcid.org/0000-0002-9695-1227
최성은(Choi, Sung Eun) ORCID logo https://orcid.org/0000-0002-6955-658X
최훈영(Choi, Hoon Young) ORCID logo https://orcid.org/0000-0002-4245-0339
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160422
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