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Changes in telomerase activity due to alternative splicing of human telomerase reverse transcriptase in colorectal cancer

Authors
 HEI CHEUL JEUNG  ;  SUN YOUNG RHA  ;  SANG JOON SHIN  ;  JOONG BAE AHN  ;  KYU HYUN PARK  ;  TAE SOO KIM  ;  JIN JU KIM  ;  JAE KYUNG ROH  ;  HYUN CHEOL CHUNG 
Citation
 Oncology Letters, Vol.14(2) : 2385-2392, 2017 
Journal Title
 Oncology Letters 
ISSN
 1792-1074 
Issue Date
2017
Keywords
alternative splicing ; colorectal cancer ; full-length ; telomerase activity ; β variant
Abstract
Human telomerase reverse transcriptase (hTERT) expression level may not always correlate with telomerase activity. The present study analyzed hTERT splicing patterns with respect to hTERT and telomerase activity in colorectal cancer. Telomerase activity was determined by telomeric repeat amplification protocol assay, and spliced variants of hTERT were identified by reverse transcription-polymerase chain reaction in 40 colorectal cancer tissue samples. In the lower range of telomerase activity (0-100 units), the percentage of the β variant decreased with the increment in telomerase activity, whereas in the higher range of telomerase activity (>100 units), total hTERT expression level revealed a trend toward increment. There was a positive correlation between the full-length variant level and β variant level. Conversely, there was a negative correlation between the percentage of the full-length variant and β variant. Tumor-node-metastasis stage was the strongest prognostic factor in multivariate analysis and the percentage of the full-length variant was an independent prognostic factor for survival. Telomerase activity was primarily altered with changes in alternative splicing of the full-length and β variants of hTERT in colorectal cancer.
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DOI
10.3892/ol.2017.6438
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
Yonsei Authors
김태수(Kim, Tae Soo)
노재경(Roh, Jae Kyung)
라선영(Rha, Sun Young)
박규현(Park, Kyu Hyun)
신상준(Shin, Sang Joon)
안중배(Ahn, Joong Bae)
정현철(Chung, Hyun Cheol) ORCID logo https://orcid.org/0000-0002-0920-9471
정희철(Jeung, Hei Cheul)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160325
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