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TLR2 contributes to trigger immune response of pleural mesothelial cells against Mycobacterium bovis BCG and M. tuberculosis infection

Authors
 Eun-Ha Hwang  ;  Tae-Hyoun Kim  ;  Ji-Yeon Park  ;  Jung Joo Hong  ;  Dong-Hyun Kim  ;  Sang-Jun Ha  ;  Soo-Jin Yang  ;  Sung Jae Shin  ;  Jong-Hwan Park 
Citation
 CYTOKINE, Vol.95 : 80-87, 2017 
Journal Title
CYTOKINE
ISSN
 1043-4666 
Issue Date
2017
MeSH
Animals ; Cell Adhesion Molecules/metabolism ; Cells, Cultured ; Chemokines/biosynthesis ; Cytokines/biosynthesis ; Epithelial Cells/enzymology ; Epithelial Cells/immunology* ; Epithelial Cells/metabolism ; Immunity, Innate ; Mice, Inbred C57BL ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Mycobacterium bovis* ; Mycobacterium tuberculosis* ; NF-kappa B/metabolism ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/biosynthesis ; Pleura/cytology* ; Signal Transduction ; Toll-Like Receptor 2/metabolism ; Toll-Like Receptor 2/physiology*
Keywords
Innate immune response ; Mycobacterium tuberculosis ; Pleural mesothelial cells ; TLR2
Abstract
Mycobacterium tuberculosis is a causative agent leading to pleural effusion, characterized by the accumulation of fluid and immune cells in the pleural cavity. Although this phenomenon has been described before, detailed processes or mechanisms associated with the pleural effusion are still not well understood. Pleural mesothelial cells (PMCs) are specialized epithelial cells that cover the body wall and internal organs in pleural cavity playing a central role in pleural inflammation. Toll-like receptors are expressed in various cell types including mesothelial cells and initiate the recognition and defense against mycobacterial infection. In the present study, we investigated direct immune responses of PMCs against two mycobacterial strains, M. bovis vaccine strain Bacille Calmette-Guérin (BCG) and M. tuberculosis virulent strain H37Rv, and the role of TLR2 in such responses. Infection with BCG and H37Rv increased the production of IL-6, CXCL1, and CCL2 in WT PMCs, which was partially impaired in TLR2-deficient cells. In addition, the activation of NF-κB and MAPKs induced by BCG and H37Rv was suppressed in TLR2-deficient PMCs, as compared with the WT cells. TLR2 deficiency led to the decrease of nitric oxide (NO) production through the delayed gene expression of iNOS in PMCs. TLR2 was also shown to be essential for optimal expression of cellular adhesion molecules such as ICAM-1 and VCAM-1 in PMCs in response to BCG and H37Rv. These findings strongly suggest that TLR2 participates in mycobacteria-induced innate immune responses in PMCs and may play a role in pathogenesis of tuberculosis pleural effusion.
Full Text
https://www.sciencedirect.com/science/article/pii/S1043466617300571
DOI
10.1016/j.cyto.2017.02.021
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Sung Jae(신성재) ORCID logo https://orcid.org/0000-0003-0854-4582
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160226
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