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House dust mite allergen Der f 2 induces interleukin-13 expression by activating the PI3K/Akt pathway

 Eun Ji Ro  ;  Pu-Hyeon Cha  ;  Hyun-Yi Kim  ;  Yong-Hee Cho  ;  Jung-Won Park  ;  Joong-Soo Han  ;  Kang-Yell Choi 
 IMMUNOLOGIC RESEARCH, Vol.56(1) : 181-188, 2013 
Journal Title
Issue Date
Animals ; Antigens, Dermatophagoides/immunology* ; Arthropod Proteins/immunology* ; Asthma/immunology* ; Cell Line ; Gene Expression Regulation/immunology ; Humans ; Immunoglobulin E/blood ; Interleukin-13/genetics ; Interleukin-13/metabolism* ; Male ; Mice ; Mice, Inbred BALB C ; NF-kappa B/metabolism ; Oncogene Protein v-akt/metabolism ; Phosphatidylinositol 3-Kinases/metabolism* ; Pyroglyphidae/immunology ; Respiratory Mucosa/immunology* ; Signal Transduction/immunology ; Transcriptional Activation/immunology
House dust mites (HDMs) are a common cause of allergic asthma. The group 2 allergen from Dermatophagoides farinae, Der f 2, is one of the major HDM allergens. Elevated Der f 2 immunoglobulin E (IgE) levels are observed in most of the allergic patients. Interleukin-13 (IL-13), a gene associated with asthma pathology, was induced by Der f 2 in BEAS-2B human airway epithelial cells; however, the signaling pathways associated with Der f 2 are not fully understood. In this study, we identified a role of the phosphatidylinositol-3-kinase (PI3K)/Akt pathway, a well-known potential target for anti-asthma drugs, in the IL-13 induction by Der f 2. First, Der f 2 activated the PI3K/Akt pathway, which subsequently activated the nuclear factor-kappa B (NF-κB) pathway and induced IL-13 expression in BEAS-2B cells. Treatment with the PI3K inhibitor LY294002 abolished Der f 2-induced activation of Akt and NF-κB and the expression of IL-13. Furthermore, Der f 2-induced activation of the PI3K/Akt and NF-κB pathways, expression of IL-13, and the blockade of these effects with a PI3K inhibitor were confirmed in the lungs of mice that were intranasally exposed to Der f 2. Taken together, these results indicate that the PI3K/Akt pathway regulates Der f 2-induced IL-13 expression via activation of the NF-κB pathway.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
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