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Chitosan/TPP-hyaluronic acid nanoparticles: a new vehicle for gene delivery to the spinal cord

Authors
 So-Jung Gwak  ;  Jong Kwon Jung  ;  Sung Su An  ;  Hyo Jin Kim  ;  Jin Soo Oh  ;  William A. Pennant  ;  Hye Yeong Lee  ;  Min Ho Kong  ;  Keung Nyun Kim  ;  Do Heum Yoon  ;  Yoon Ha 
Citation
 JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, Vol.23(11) : 1437-1450, 2012 
Journal Title
 JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 
ISSN
 0920-5063 
Issue Date
2012
MeSH
Animals ; Cell Survival/physiology ; Cells, Cultured ; Chitosan*/chemistry ; DNA/administration & dosage* ; DNA/chemistry ; Disease Models, Animal ; Gene Transfer Techniques*/instrumentation ; Heterocyclic Compounds*/chemistry ; Hyaluronic Acid*/chemistry ; Male ; Mice ; Nanoparticles*/chemistry ; Neural Stem Cells/physiology ; Organophosphorus Compounds*/chemistry ; Rats, Sprague-Dawley ; Spinal Cord/physiology ; Spinal Cord/physiopathology ; Spinal Cord Injuries/physiopathology ; Spinal Cord Injuries/therapy ; Tissue Culture Techniques
Keywords
Chitosan ; gene delivery ; spinal cord
Abstract
Gene delivery offers therapeutic promise for the treatment of neurological diseases and spinal cord injury. Several studies have offered viral vectors as vehicles to deliver therapeutic agents, yet their toxicity and immunogenicity, along with the cost of their large-scale formulation, limits their clinical use. As such, non-viral vectors are attractive in that they offer improved safety profiles compared to viruses. Poly(ethylene imine) (PEI) is one of the most extensively studied non-viral vectors, but its clinical value is limited y its cytotoxicity. Recently, chitosan/DNA complex nanoparticles have een considered as a vector for gene delivery. Here, we demonstrate that DNA nanoparticles made of hyaluronic acid (HA) and chitosan have low cytotoxicity and induce high transgene expression in neural stem cells and organotypic spinal cord slice tissue. Chitosan-TPP/HA nanoparticles were significantly less cytotoxic than PEI at various concentrations. Additionally, chitosan-TPP/HA nanoparticles with pDNA induced higher transgene expression in vitro for a longer duration than PEI in neural stem cells. These results suggest chitosan-TPP/HA nanoparticles may have the potential to serve as an option for gene delivery to the spinal cord.
Full Text
http://www.ingentaconnect.com/content/tandf/bsp/2012/00000023/00000011/art00004
DOI
10.1163/092050611X584090
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Keung Nyun(김긍년)
Oh, Jin Soo(오진수)
Yoon, Do Heum(윤도흠) ORCID logo https://orcid.org/0000-0003-1452-5724
Ha, Yoon(하윤)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/158325
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