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Comparison between mixed lysate antigen and α-actinin antigen in ELISA for serodiagnosis of trichomoniasis

Authors
 Seung-Ryong Kim  ;  Jung-Hyun Kim  ;  Soon-Jung Park  ;  Hye-Yeon Lee  ;  Yong-Suk Kim  ;  Yu-Mi Kim  ;  Yeon-Chul Hong  ;  Jae-Sook Ryu 
Citation
 PARASITOLOGY INTERNATIONAL, Vol.64(5) : 405-407, 2015 
Journal Title
PARASITOLOGY INTERNATIONAL
ISSN
 1383-5769 
Issue Date
2015
MeSH
Actinin/immunology* ; Adult ; Aged ; Aged, 80 and over ; Animals ; Antibodies, Protozoan/blood ; Antigens, Protozoan/immunology* ; Case-Control Studies ; Clinical Laboratory Techniques/methods ; Enzyme-Linked Immunosorbent Assay/methods* ; Female ; Humans ; Male ; Middle Aged ; Trichomonas Vaginitis/blood ; Trichomonas Vaginitis/diagnosis* ; Trichomonas vaginalis/genetics ; Trichomonas vaginalis/immunology*
Keywords
ELISA ; Lysate ; Recombinant α-actinin ; Serodiagnosis ; Trichomonas vaginalis
Abstract
The aim of this study was to identify an antigen suitable for ELISA for serodiagnosis of Trichomonas vaginalis (T. vaginalis) infection. Mixed lysate antigen (Ag) from eight strains of T. vaginalis and recombinant α-actinin protein was compared. The sera of three groups were examined by ELISA: 73 women infected with trichomoniasis served as a positive control, 31 male volunteers as a negative control, and 424 women attending an outpatient health screening at Hanyang University Guri Hospital. Based on the cutoff optical density for each Ag obtained with a negative control, the serosensitivity of the mixed lysate Ag (79.5%) was significantly higher than that of the α-actinin (52.1%) in the 73 patients with trichomoniasis. The specificity using lysate Ag and α-actinin was 100% and 96.8%, respectively. On the other hand, the positivity rate in 424 outpatients was 39.2% and 11.8% with mixed lysate and α-actinin Ag, respectively. Taken together, mixed lysate Ag showed higher sensitivity and specificity than α-actinin. Therefore, mixed lysate may be a better Ag than α-actinin for ELISA for the diagnosis of trichomoniasis.
Full Text
http://www.sciencedirect.com/science/article/pii/S1383576915001002
DOI
10.1016/j.parint.2015.06.003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Lee, Hye Yeon(이혜연)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/157061
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