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Complete genome sequence of the siphoviral bacteriophage B/-R3177, which lyses an OXA-66-producing carbapenem-resistant Acinetobacter baumannii isolate

Authors
 Jongsoo Jeon  ;  Roshan D’Souza  ;  Naina Pinto  ;  Choong-Min Ryu  ;  Jong-hwan Park  ;  Dongeun Yong  ;  Kyungwon Le 
Citation
 ARCHIVES OF VIROLOGY, Vol.160(12) : 3157-3160, 2015 
Journal Title
 ARCHIVES OF VIROLOGY 
ISSN
 0304-8608 
Issue Date
2015
MeSH
Acinetobacter baumannii/drug effects ; Acinetobacter baumannii/enzymology ; Acinetobacter baumannii/virology* ; Bacterial Proteins/metabolism ; Bacteriophages/classification ; Bacteriophages/genetics* ; Bacteriophages/isolation & purification* ; Bacteriophages/physiology ; Base Sequence ; Carbapenems/pharmacology ; Drug Resistance, Bacterial ; Genome, Viral* ; Molecular Sequence Data ; Open Reading Frames ; Phylogeny ; Siphoviridae/classification ; Siphoviridae/genetics* ; Siphoviridae/isolation & purification* ; Siphoviridae/physiology ; beta-Lactamases/metabolism
Keywords
Baumannii Strain ; Putative Phage ; Acinetobacter Baumannii Isolate ; Acinetobacter Phage ; Putative tRNA Gene
Abstract
In recent years, antimicrobial resistance has become a major medical threat worldwide. Among these threats, the rapid increase in carbapenem-resistant Acinetobacter baumannii (CRAB) is a particularly challenging global issue in the health care setting. In this study, a novel lytic A. baumannii phage, B/-R3177, infecting carbapenem-resistant A. baumannii strains was isolated from sewage samples at a hospital. The morphology of the phage as assessed by transmission electron microscopy (TEM) indicated that it belongs to the family Siphoviridae within the order Caudovirales. It has a linear double-stranded DNA genome of 47,575 bp with a G?C content of 39.83 %. Eighty open reading frames (ORFs) were predicted; however, only 14 ORFs were annotated as encoding functional proteins, while most of the ORFs encoded hypothetical proteins. Among the total ORFs of the phage genome, no toxin-related genes were detected. A bioinformatics analysis showed that the whole genome sequence of phage B/-R3177 exhibited 62 % sequence similarity to that of Acinetobacter phage B/-B1252, but there was no homology seen with other phages. Physiological characteristics, such as one-step growth properties, pH and temperature stability, and host cell lysis activity showed this phage has high stability and lytic activity against host bacteria and therefore has potential applicability as an antibacterial agent to control pathogens in the hospital environment.
Full Text
https://link.springer.com/article/10.1007%2Fs00705-015-2604-y
DOI
10.1007/s00705-015-2604-y
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeon, Jong Soo(전종수) ORCID logo https://orcid.org/0000-0002-7213-5663
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156899
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