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MTB-DR-RIF 9G test: Detection and discrimination of tuberculosis and multi-drug resistant tuberculosis strains

Authors
 Keum-Soo Song  ;  Satish Balasaheb Nimse  ;  Nam Hoon Cho  ;  Nackmoon Sung  ;  Hee-jin Kim  ;  Jeongseong Yang  ;  Taisun Kim 
Citation
 TUBERCULOSIS, Vol.95(6) : 780-785, 2015 
Journal Title
 TUBERCULOSIS 
ISSN
 1472-9792 
Issue Date
2015
MeSH
Antibiotics, Antitubercular/therapeutic use* ; Bacterial Proteins/genetics* ; Bacteriological Techniques* ; DNA Mutational Analysis ; DNA, Bacterial/genetics* ; DNA-Directed RNA Polymerases/genetics* ; Diagnosis, Differential ; Discriminant Analysis ; Drug Resistance, Multiple, Bacterial/genetics* ; Humans ; Molecular Diagnostic Techniques* ; Mutation ; Mycobacterium tuberculosis/drug effects ; Mycobacterium tuberculosis/genetics* ; Nucleic Acid Hybridization ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Predictive Value of Tests ; Reproducibility of Results ; Rifampin/therapeutic use* ; Tuberculosis/diagnosis* ; Tuberculosis/drug therapy ; Tuberculosis/microbiology ; Tuberculosis, Multidrug-Resistant/diagnosis* ; Tuberculosis, Multidrug-Resistant/drug therapy ; Tuberculosis, Multidrug-Resistant/microbiology
Keywords
Accuracy ; Diagnosis ; Drug resistant tuberculosis ; Sensitivity ; Specificity ; Tuberculosis
Abstract
This report describes the evaluation of the novel MTB-DR-RIF 9G test for the accurate detection and discrimination of Mycobacterium tuberculosis (MTB) and rifampicin-resistant M. tuberculosis (MTB-DR-RIF) in the clinical samples. The procedure included the amplification of a nucleotide fragment of the rpoB gene of the MTB and MTB-DR-RIF strains and their hybridization with the immobilized probes. The MTB-DR-RIF 9G test was evaluated for its ability to detect and discriminate MTB and MTB-DR-RIF strains in 113 known clinical samples. The accuracy of the MTB-DR-RIF 9G test was determined by comparing its results with sequencing analysis and drug susceptibility testing. The sensitivity and specificity of the MTB-DR-RIF 9G test at 95% confidence interval were found to be 95.4% (89.5-98.5) and 100% (69.2-100), respectively. The positive predictive value and negative predictive value of the MTB-DR-RIF 9G test at 95% confidence interval were found to be 100% (85.0-95.9) and 66.7% (38.4-88.18), respectively. Sequencing analysis of all samples indicated that the mutations present in the regions identified with the MTB-DR-RIF 9G assay can be detected accurately.
Full Text
http://www.sciencedirect.com/science/article/pii/S1472979215301335
DOI
10.1016/j.tube.2015.08.008
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156883
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