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MTB-DR-RIF 9G test: Detection and discrimination of tuberculosis and multi-drug resistant tuberculosis strains

DC FieldValueLanguage
dc.contributor.author조남훈-
dc.date.accessioned2018-03-26T16:51:37Z-
dc.date.available2018-03-26T16:51:37Z-
dc.date.issued2015-
dc.identifier.issn1472-9792-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/156883-
dc.description.abstractThis report describes the evaluation of the novel MTB-DR-RIF 9G test for the accurate detection and discrimination of Mycobacterium tuberculosis (MTB) and rifampicin-resistant M. tuberculosis (MTB-DR-RIF) in the clinical samples. The procedure included the amplification of a nucleotide fragment of the rpoB gene of the MTB and MTB-DR-RIF strains and their hybridization with the immobilized probes. The MTB-DR-RIF 9G test was evaluated for its ability to detect and discriminate MTB and MTB-DR-RIF strains in 113 known clinical samples. The accuracy of the MTB-DR-RIF 9G test was determined by comparing its results with sequencing analysis and drug susceptibility testing. The sensitivity and specificity of the MTB-DR-RIF 9G test at 95% confidence interval were found to be 95.4% (89.5-98.5) and 100% (69.2-100), respectively. The positive predictive value and negative predictive value of the MTB-DR-RIF 9G test at 95% confidence interval were found to be 100% (85.0-95.9) and 66.7% (38.4-88.18), respectively. Sequencing analysis of all samples indicated that the mutations present in the regions identified with the MTB-DR-RIF 9G assay can be detected accurately.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherChurchill Livingstone-
dc.relation.isPartOfTUBERCULOSIS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAntibiotics, Antitubercular/therapeutic use*-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHBacteriological Techniques*-
dc.subject.MESHDNA Mutational Analysis-
dc.subject.MESHDNA, Bacterial/genetics*-
dc.subject.MESHDNA-Directed RNA Polymerases/genetics*-
dc.subject.MESHDiagnosis, Differential-
dc.subject.MESHDiscriminant Analysis-
dc.subject.MESHDrug Resistance, Multiple, Bacterial/genetics*-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Diagnostic Techniques*-
dc.subject.MESHMutation-
dc.subject.MESHMycobacterium tuberculosis/drug effects-
dc.subject.MESHMycobacterium tuberculosis/genetics*-
dc.subject.MESHNucleic Acid Hybridization-
dc.subject.MESHPolymerase Chain Reaction-
dc.subject.MESHPolymorphism, Single Nucleotide-
dc.subject.MESHPredictive Value of Tests-
dc.subject.MESHReproducibility of Results-
dc.subject.MESHRifampin/therapeutic use*-
dc.subject.MESHTuberculosis/diagnosis*-
dc.subject.MESHTuberculosis/drug therapy-
dc.subject.MESHTuberculosis/microbiology-
dc.subject.MESHTuberculosis, Multidrug-Resistant/diagnosis*-
dc.subject.MESHTuberculosis, Multidrug-Resistant/drug therapy-
dc.subject.MESHTuberculosis, Multidrug-Resistant/microbiology-
dc.titleMTB-DR-RIF 9G test: Detection and discrimination of tuberculosis and multi-drug resistant tuberculosis strains-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Pathology-
dc.contributor.googleauthorKeum-Soo Song-
dc.contributor.googleauthorSatish Balasaheb Nimse-
dc.contributor.googleauthorNam Hoon Cho-
dc.contributor.googleauthorNackmoon Sung-
dc.contributor.googleauthorHee-jin Kim-
dc.contributor.googleauthorJeongseong Yang-
dc.contributor.googleauthorTaisun Kim-
dc.identifier.doi10.1016/j.tube.2015.08.008-
dc.contributor.localIdA03812-
dc.relation.journalcodeJ02760-
dc.identifier.eissn1873-281X-
dc.identifier.pmid26381057-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1472979215301335-
dc.subject.keywordAccuracy-
dc.subject.keywordDiagnosis-
dc.subject.keywordDrug resistant tuberculosis-
dc.subject.keywordSensitivity-
dc.subject.keywordSpecificity-
dc.subject.keywordTuberculosis-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.citation.volume95-
dc.citation.number6-
dc.citation.startPage780-
dc.citation.endPage785-
dc.identifier.bibliographicCitationTUBERCULOSIS, Vol.95(6) : 780-785, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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