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Effect of dentin treatment on proliferation and differentiation of human dental pulp stem cells

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dc.contributor.author방난심-
dc.contributor.author정일영-
dc.date.accessioned2018-03-26T16:47:25Z-
dc.date.available2018-03-26T16:47:25Z-
dc.date.issued2015-
dc.identifier.issn2234-7658-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/156819-
dc.description.abstractOBJECTIVES: Sodium hypochlorite (NaOCl) is an excellent bactericidal agent, but it is detrimental to stem cell survival, whereas intracanal medicaments such as calcium hydroxide (Ca[OH]2) promote the survival and proliferation of stem cells. This study evaluated the effect of sequential NaOCl and Ca[OH]2 application on the attachment and differentiation of dental pulp stem cells (DPSCs). MATERIALS AND METHODS: DPSCs were obtained from human third molars. All dentin specimens were treated with 5.25% NaOCl for 30 min. DPSCs were seeded on the dentin specimens and processed with additional 1 mg/mL Ca[OH]2, 17% ethylenediaminetetraacetic acid (EDTA) treatment, file instrumentation, or a combination of these methods. After 7 day of culture, we examined DPSC morphology using scanning electron microscopy and determined the cell survival rate with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We measured cell adhesion gene expression levels after 4 day of culture and odontogenic differentiation gene expression levels after 4 wk using quantitative real-time polymerase chain reaction. RESULTS: DPSCs did not attach to the dentin in the NaOCl-treated group. The gene expression levels of fibronectin-1 and secreted phosphoprotein-1 gene in both the Ca[OH]2- and the EDTA-treated groups were significantly higher than those in the other groups. All Ca[OH]2-treated groups showed higher expression levels of dentin matrix protein-1 than that of the control. The dentin sialophosphoprotein level was significantly higher in the groups treated with both Ca[OH]2 and EDTA. CONCLUSIONS: The application of Ca[OH]2 and additional treatment such as EDTA or instrumentation promoted the attachment and differentiation of DPSCs after NaOCl treatment.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean-
dc.publisherKorean Academy of Conservative Dentistry-
dc.relation.isPartOfRestorative Dentistry & Endodontics-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEffect of dentin treatment on proliferation and differentiation of human dental pulp stem cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry-
dc.contributor.departmentDept. of Advanced General Dentistry-
dc.contributor.googleauthorMinjeong Park-
dc.contributor.googleauthorNan-Sim Pang-
dc.contributor.googleauthorIl-Young Jung-
dc.identifier.doi10.5395/rde.2015.40.4.290-
dc.contributor.localIdA01783-
dc.contributor.localIdA03700-
dc.relation.journalcodeJ02618-
dc.identifier.eissn2234-7666-
dc.identifier.pmid26587415-
dc.subject.keywordCalcium hydroxide-
dc.subject.keywordCell attachment-
dc.subject.keywordCell differentiation-
dc.subject.keywordDental pulp stem cells-
dc.subject.keywordRegenerative endodontics-
dc.subject.keywordSodium hypochlorite-
dc.contributor.alternativeNamePang, Nan Sim-
dc.contributor.alternativeNameJung, Il Young-
dc.contributor.affiliatedAuthorPang, Nan Sim-
dc.contributor.affiliatedAuthorJung, Il Young-
dc.citation.volume40-
dc.citation.number4-
dc.citation.startPage290-
dc.citation.endPage298-
dc.identifier.bibliographicCitationRestorative Dentistry & Endodontics, Vol.40(4) : 290-298, 2015-
dc.identifier.rimsid39947-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers

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