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Synergistic Effects of a Calcium Phosphate/Fibronectin Coating on the Adhesion of Periodontal Ligament Stem Cells Onto Decellularized Dental Root Surfaces

Authors
 Jung-Seok Lee  ;  Hyun-Suk Kim  ;  So-Yon Park  ;  Tae-Wan Kim  ;  Jae-Suk Jung  ;  Jong-Bin Lee  ;  Chang-Sung Kim 
Citation
 CELL TRANSPLANTATION, Vol.24(9) : 1767-1779, 2015 
Journal Title
 CELL TRANSPLANTATION 
ISSN
 0963-6897 
Issue Date
2015
MeSH
Animals ; Antigens, CD/metabolism ; Calcium Phosphates/chemistry* ; Cell Adhesion ; Cells, Cultured ; Dogs ; Fibronectins/chemistry* ; Male ; Periodontal Ligament/cytology* ; Stem Cell Transplantation ; Stem Cells/cytology* ; Stem Cells/metabolism ; Tooth/pathology ; Tooth Root/cytology ; Tooth Root/diagnostic imaging ; Tooth Root/metabolism ; Transplantation, Autologous ; X-Ray Microtomography
Keywords
Autophagy ; Chemotherapy ; Hypoxia ; Lung cancer ; Resistance
Abstract
This study aimed to enhance the attachment of periodontal ligament stem cells (PDLSCs) onto the decellularized dental root surface using surface coating with fibronectin and/or calcium phosphate (CaP) and to evaluate the activity of PDLSCs attached to a coated dental root surface following tooth replantation. PDLSCs were isolated from five dogs, and the other dental roots were used as a scaffold for carrying PDLSCs and then assigned to one of four groups according to whether their surface was coated with CaP, fibronectin, CaP/fibronectin, or left uncoated (control). Fibronectin increased the adhesion of PDLSCs onto dental root surfaces compared to both the control and CaP-coated groups, and simultaneous surface coating with CaP and fibronectin significantly accelerated and increased PDLSC adhesion compared to the fibronectin-only group. On in vivo tooth replantation, functionally oriented periodontal new attachment was observed on the CaP/fibronectin-coated dental roots to which autologous PDLSCs had adhered, while in the control condition, dental root replantation was associated only with root resorption and ankylosis along the entire root length. CaP and fibronectin synergistically enhanced the attachment of PDLSCs onto dental root surfaces, and autologous PDLSCs could produce de novo periodontal new attachment in an experimental in vivo model.
Full Text
http://www.ingentaconnect.com/contentone/cog/ct/2015/00000024/00000009/art00008
DOI
10.3727/096368914X684628
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Kim, Tae-Wan(김태완)
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156756
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