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Bone Tissue Engineering by Using Calcium Phosphate Glass Scaffolds and the Avidin-Biotin Binding System

Authors
 Min-Chul Kim  ;  Min-Ho Hong  ;  Byung-Hyun Lee  ;  Heon-Jin Choi  ;  Yeong-mu Ko  ;  Yong-Keun Lee 
Citation
 ANNALS OF BIOMEDICAL ENGINEERING, Vol.43(12) : 3004-3014, 2015 
Journal Title
 ANNALS OF BIOMEDICAL ENGINEERING 
ISSN
 0090-6964 
Issue Date
2015
MeSH
Avidin/chemistry ; Biotin/chemistry ; Bone and Bones* ; Calcium Phosphates/chemistry ; Cell Adhesion ; Cell Differentiation ; Cell Line ; Cell Proliferation ; Glass/chemistry ; Humans ; Polyurethanes/chemistry ; Tissue Engineering/methods* ; Tissue Scaffolds*
Keywords
Avidin–biotin binding system ; Bone tissue engineering ; Calcium phosphate glass ; Scaffold
Abstract
Highly porous and interconnected scaffolds were fabricated using calcium phosphate glass (CPG) for bone tissue engineering. An avidin-biotin binding system was used to improve osteoblast-like cell adhesion to the scaffold. The scaffolds had open macro- and micro-scale pores, and continuous struts without cracks or defects. Scaffolds prepared using a mixture (amorphous and crystalline CPG) were stronger than amorphous group and crystalline group. Cell adhesion assays showed that more cells adhered, with increasing cell seeding efficiency to the avidin-adsorbed scaffolds, and that cell attachment to the highly porous scaffolds significantly differed between avidin-adsorbed scaffolds and other scaffolds. Proliferation was also significantly higher for avidin-adsorbed scaffolds. Osteoblastic differentiation of MG-63 cells was observed at 3 days, and MG-63 cells in direct contact with avidin-adsorbed scaffolds were positive for type I collagen, osteopontin, and alkaline phosphatase gene expression. Osteocalcin expression was observed in the avidin-adsorbed scaffolds at 7 days, indicating that cell differentiation in avidin-adsorbed scaffolds occurred faster than the other scaffolds. Thus, these CPG scaffolds have excellent biological properties suitable for use in bone tissue engineering.
Full Text
https://link.springer.com/article/10.1007/s10439-015-1347-y
DOI
10.1007/s10439-015-1347-y
Appears in Collections:
5. Research Institutes (연구소) > Others (기타) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kim, Min Chul(김민철)
Lee, Byung Hyun(이병현)
Hong, Min Ho(홍민호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156703
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