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Bone Tissue Engineering by Using Calcium Phosphate Glass Scaffolds and the Avidin-Biotin Binding System

DC Field Value Language
dc.contributor.author김민철-
dc.contributor.author이병현-
dc.date.accessioned2018-03-26T16:40:45Z-
dc.date.available2018-03-26T16:40:45Z-
dc.date.issued2015-
dc.identifier.issn0090-6964-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/156703-
dc.description.abstractHighly porous and interconnected scaffolds were fabricated using calcium phosphate glass (CPG) for bone tissue engineering. An avidin-biotin binding system was used to improve osteoblast-like cell adhesion to the scaffold. The scaffolds had open macro- and micro-scale pores, and continuous struts without cracks or defects. Scaffolds prepared using a mixture (amorphous and crystalline CPG) were stronger than amorphous group and crystalline group. Cell adhesion assays showed that more cells adhered, with increasing cell seeding efficiency to the avidin-adsorbed scaffolds, and that cell attachment to the highly porous scaffolds significantly differed between avidin-adsorbed scaffolds and other scaffolds. Proliferation was also significantly higher for avidin-adsorbed scaffolds. Osteoblastic differentiation of MG-63 cells was observed at 3 days, and MG-63 cells in direct contact with avidin-adsorbed scaffolds were positive for type I collagen, osteopontin, and alkaline phosphatase gene expression. Osteocalcin expression was observed in the avidin-adsorbed scaffolds at 7 days, indicating that cell differentiation in avidin-adsorbed scaffolds occurred faster than the other scaffolds. Thus, these CPG scaffolds have excellent biological properties suitable for use in bone tissue engineering.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherSpringer Science + Business Media-
dc.relation.isPartOfANNALS OF BIOMEDICAL ENGINEERING-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAvidin/chemistry-
dc.subject.MESHBiotin/chemistry-
dc.subject.MESHBone and Bones*-
dc.subject.MESHCalcium Phosphates/chemistry-
dc.subject.MESHCell Adhesion-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Line-
dc.subject.MESHCell Proliferation-
dc.subject.MESHGlass/chemistry-
dc.subject.MESHHumans-
dc.subject.MESHPolyurethanes/chemistry-
dc.subject.MESHTissue Engineering/methods*-
dc.subject.MESHTissue Scaffolds*-
dc.titleBone Tissue Engineering by Using Calcium Phosphate Glass Scaffolds and the Avidin-Biotin Binding System-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry-
dc.contributor.departmentOthers-
dc.contributor.googleauthorMin-Chul Kim-
dc.contributor.googleauthorMin-Ho Hong-
dc.contributor.googleauthorByung-Hyun Lee-
dc.contributor.googleauthorHeon-Jin Choi-
dc.contributor.googleauthorYeong-mu Ko-
dc.contributor.googleauthorYong-Keun Lee-
dc.identifier.doi10.1007/s10439-015-1347-y-
dc.contributor.localIdA05227-
dc.contributor.localIdA02799-
dc.contributor.localIdA04392-
dc.relation.journalcodeJ00154-
dc.identifier.eissn1573-9686-
dc.identifier.pmid26040755-
dc.identifier.urlhttps://link.springer.com/article/10.1007/s10439-015-1347-y-
dc.subject.keywordAvidin–biotin binding system-
dc.subject.keywordBone tissue engineering-
dc.subject.keywordCalcium phosphate glass-
dc.subject.keywordScaffold-
dc.contributor.alternativeNameKim, Min Chul-
dc.contributor.alternativeNameLee, Byung Hyun-
dc.contributor.affiliatedAuthorKim, Min Chul-
dc.contributor.affiliatedAuthorLee, Byung Hyun-
dc.citation.volume43-
dc.citation.number12-
dc.citation.startPage3004-
dc.citation.endPage3014-
dc.identifier.bibliographicCitationANNALS OF BIOMEDICAL ENGINEERING, Vol.43(12) : 3004-3014, 2015-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers

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