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Donor pretreatment with carbon monoxide prevents ischemia/reperfusion injury following heart transplantation in rats

 Noritomo Fujisaki  ;  Keisuke Kohama  ;  Takeshi Nishimura  ;  Hayato Yamashita  ;  Michiko Ishikawa  ;  Akihiro Kanematsu  ;  Taihei Yamada  ;  Sungsoo Lee  ;  Tetsuya Yumoto  ;  Kohei Tsukahara  ;  Joji Kotani  ;  Atsunori Nakao 
 MEDICAL GAS RESEARCH, Vol.6(3) : 122-129, 2016 
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Because inhaled carbon monoxide (CO) provides potent anti-inflammatory and antioxidant effects against ischemia reperfusion injury, we hypothesized that treatment of organ donors with inhaled CO would decrease graft injury after heart transplantation. Hearts were heterotopically transplanted into syngeneic Lewis rats after 8 hours of cold preservation in University of Wisconsin solution. Donor rats were exposed to CO at a concentration of 250 parts per million for 24 hours via a gas-exposure chamber. Severity of myocardial injury was determined by total serum creatine phosphokinase and troponin I levels at three hours after reperfusion. In addition, Affymetrix gene array analysis of mRNA transcripts was performed on the heart graft tissue prior to implantation. Recipients of grafts from CO-exposed donors had lower levels of serum troponin I and creatine phosphokinase; less upregulation of mRNA for interleukin-6, intercellular adhesion molecule-1, and tumor necrosis factor-α; and fewer infiltrating cells. Although donor pretreatment with CO altered the expression of 49 genes expressly represented on the array, we could not obtain meaningful data to explain the mechanisms by which CO potentiated the protective effects. Pretreatment with CO gas before organ procurement effectively protected cardiac grafts from ischemia reperfusion-induced injury in a rat heterotopic cardiac transplant model. A clinical report review indicated that CO-poisoned organ donors may be comparable to non-poisoned donors.
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1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Sung Soo(이성수) ORCID logo https://orcid.org/0000-0001-8998-9510
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