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Characterization and complete genome sequence analysis of two Myoviral bacteriophages infecting clinical carbapenem-resistant Acinetobacter baumannii isolates

Authors
 J. Jeon  ;  R. D’Souza  ;  N. Pinto  ;  C.-M. Ryu  ;  J. Park  ;  D. Yong  ;  K. Lee 
Citation
 Journal of Applied Microbiology, Vol.121(1) : 68-77, 2016 
Journal Title
 Journal of Applied Microbiology 
Issue Date
2016
MeSH
Acinetobacter baumannii/drug effects ; Acinetobacter baumannii/isolation & purification ; Acinetobacter baumannii/virology* ; Anti-Bacterial Agents/pharmacology ; Bacteriophages/genetics* ; Bacteriophages/isolation & purification ; Bacteriophages/ultrastructure ; Carbapenems/pharmacology ; DNA, Viral/chemistry ; Drug Resistance, Bacterial ; Genome, Viral* ; Humans ; Open Reading Frames ; Sequence Analysis, DNA
Keywords
Acinetobacter baumannii ; Myoviridae ; antimicrobial alternatives ; bacteriophage ; biocontrol ; carbapenem
Abstract
AIMS: The aim of this study was to characterize phenotypical properties, to analyse whole genomes of novel Acinetobacter baumannii phages infecting carbapenem-resistant Ac. baumannii (CRAB) and to evaluate their potential as antimicrobial alternatives to control Ac. baumannii in clinical settings. METHODS AND RESULTS: The Ac. baumannii phages, Βϕ-R1215 and Βϕ-R2315, were isolated from sewage samples. These phages were characterized by transmission electron microscopy, host spectrum, the thermal/pH stability test, the bacterial lysis assay and the whole genome analysis. Both phages lysed 21 of 45 CRAB hosts, and showed high stability at various pH (pH 4-10) and temperature (25-60°C), and were strongly active against host bacteria in vitro. The genomes of Βϕ-R1215 and Βϕ-R2315 are linear double-strands of DNA with 44·866 and 44·846 bp respectively. These two genomes revealed high similarity at the DNA level, but the organization and direction of open reading frames were different. CONCLUSIONS: The Ac. baumannii phages, Βϕ-R1215 and Βϕ-R2315, are novel lytic phages lysing CRAB strains which were isolated from respiratory samples of patients. SIGNIFICANCE AND IMPACT OF THE STUDY: In vitro and in silico data showed that these novel Ac. baumannii phages, Βϕ-R1215 and Βϕ-R2315, have potential as antimicrobial alternatives to control CRAB in healthcare settings.
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DOI
10.1111/jam.13134
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
용동은(Yong, Dong Eun) ORCID logo https://orcid.org/0000-0002-1225-8477
이경원(Lee, Kyungwon) ORCID logo https://orcid.org/0000-0003-3788-2134
전종수(Jeon, Jong Soo) ORCID logo https://orcid.org/0000-0002-7213-5663
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/155747
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