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Cytotoxicity of Light-Cured Dental Materials according to Different Sample Preparation Methods

Authors
 Myung-Jin Lee  ;  Mi-Joo Kim  ;  Jae-Sung Kwon  ;  Sang-Bae Lee  ;  Kwang-Mahn Kim 
Citation
 MATERIALS, Vol.10(3) : 288, 2017 
Journal Title
 MATERIALS 
Issue Date
2017
Keywords
biocompatibility ; cytotoxicity ; oxygen-inhibition layer ; resin-based materials ; sample preparation
Abstract
Dental light-cured resins can undergo different degrees of polymerization when applied in vivo. When polymerization is incomplete, toxic monomers may be released into the oral cavity. The present study assessed the cytotoxicity of different materials, using sample preparation methods that mirror clinical conditions. Composite and bonding resins were used and divided into four groups according to sample preparation method: uncured; directly cured samples, which were cured after being placed on solidified agar; post-cured samples were polymerized before being placed on agar; and “removed unreacted layer” samples had their oxygen-inhibition layer removed after polymerization. Cytotoxicity was evaluated using an agar diffusion test, MTT assay, and confocal microscopy. Uncured samples were the most cytotoxic, while removed unreacted layer samples were the least cytotoxic (p < 0.05). In the MTT assay, cell viability increased significantly in every group as the concentration of the extracts decreased (p < 0.05). Extracts from post-cured and removed unreacted layer samples of bonding resin were less toxic than post-cured and removed unreacted layer samples of composite resin. Removal of the oxygen-inhibition layer resulted in the lowest cytotoxicity. Clinicians should remove unreacted monomers on the resin surface immediately after restoring teeth with light-curing resin to improve the restoration biocompatibility.
Files in This Item:
T201700621.pdf Download
DOI
10.3390/ma10030288
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Kim, Mi Joo(김미주)
Lee, Sang Bae(이상배)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154695
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