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Role of tropomyosin in silkworm allergy

 Kyoung Yong Jeong  ;  In‑Soo Han  ;  June Yong Lee  ;  Kyung Hee Park  ;  Jae‑Hyun Lee  ;  Jung‑Won Park 
 Molecular Medicine Reports, Vol.15(5) : 3264-3270, 2017 
Journal Title
 Molecular Medicine Reports 
Issue Date
Adolescent ; Adult ; Allergens/immunology* ; Allergens/metabolism ; Amino Acid Sequence ; Animals ; Antigen-Antibody Reactions ; Bombyx/growth & development ; Bombyx/metabolism ; Brachyura/metabolism ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Hypersensitivity/immunology ; Hypersensitivity/pathology* ; Immunoglobulin E/blood ; Immunoglobulin E/immunology ; Male ; Pupa/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/immunology ; Recombinant Proteins/isolation & purification ; Sequence Alignment ; Skin Tests ; Tropomyosin/genetics ; Tropomyosin/immunology* ; Tropomyosin/metabolism ; Young Adult
Silkworm pupae are widely consumed in Asian countries and allergic reactions following consumption have been described. However, false‑positive responses in skin prick allergy tests or non‑specific immunoglobulin E (IgE) responses to total extract of silkworm pupa make diagnosis difficult. Although improved allergy diagnosis is required, molecular characterization of silkworm allergens has not been performed to date, except for Bomb m 1, an arginine kinase. This study aimed to evaluate the allergenicity of tropomyosin, a well‑established invertebrate pan‑allergen, from silkworm pupa. The silkworm tropomyosin gene was cloned by reverse transcription and polymerase chain reaction, and the protein was overexpressed in Escherichia coli and purified by affinity chromatography using Nickel‑resin. IgE reactivity of the recombinant protein was examined by ELISA and competitive inhibition analyses. Silkworm pupa tropomyosin shared 73.5‑92.3% amino acid sequence identity with previously identified allergenic tropomyosins. Sera from eight of 15 patients with silkworm allergy (53.3%) exhibited binding of IgE to the recombinant protein. However, recombinant protein was able to inhibit less than 10% of IgE reactivity to silkworm pupa extract. Of the eight sera tested, six that specifically reacted with silkworm tropomyosin also demonstrated IgE reactivity to shrimp and crab. In the present study, specific IgE to silkworm tropomyosin was detected in patients with silkworm allergy, suggesting that it may be useful in diagnosis of allergy to silkworm pupa.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
박경희(Park, Kyung Hee) ORCID logo https://orcid.org/0000-0003-3605-5364
박중원(Park, Jung Won) ORCID logo https://orcid.org/0000-0003-0249-8749
이재현(Lee, Jae Hyun) ORCID logo https://orcid.org/0000-0002-0760-0071
정경용(Jeong, Kyoung Yong) ORCID logo https://orcid.org/0000-0001-9887-1426
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