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Adenovirus-mediated Foxp3 expression in lung epithelial cells ameliorates acute radiation-induced pneumonitis in mice

Authors
 D Shin  ;  G Lee  ;  S Lee  ;  S Park  ;  K-H Jung  ;  J H Lee  ;  J M Lee  ;  J-Y Kim  ;  J Cho  ;  H Bae 
Citation
 GENE THERAPY, Vol.24(2) : 104-112, 2017 
Journal Title
 GENE THERAPY 
ISSN
 0969-7128 
Issue Date
2017
MeSH
Adenoviridae/genetics* ; Animals ; Epithelial Cells/immunology ; Epithelial Cells/metabolism* ; Epithelial Cells/radiation effects ; Female ; Forkhead Transcription Factors/genetics* ; Genetic Therapy* ; Genetic Vectors/administration & dosage ; Lung/immunology ; Lung/metabolism* ; Lung/radiation effects ; Mice ; Mice, Inbred C57BL ; Pneumonia/etiology ; Pneumonia/prevention & control* ; Radiation Pneumonitis/etiology ; Radiation Pneumonitis/prevention & control* ; T-Lymphocytes, Regulatory/immunology ; X-Rays/adverse effects*
Abstract
Forkhead transcription factor 3 (Foxp3) has a critical role in regulatory T cells (Treg). There are an increasing number of researches concerning the functions of Foxp3 in other cells, including lung epithelial cells besides Treg. However, the roles of Foxp3 in lung epithelial cells remain poorly understood. To examine the potential therapeutic benefits of Foxp3 for lung inflammation, this study investigates the effect of adenovirus-mediated Foxp3 overexpression in a radiation-induced lung damage model. Foxp3-EGFP expressing adenovirus was administered by intratracheal injection three times over 14 days after focal X-ray irradiation. To evaluate effects of Foxp3 overexpression in radiation-induced lung inflammation, immune cell profiles of bronchoalveolar lavage (BAL) fluid were analyzed. Foxp3 gene-delivered mice showed significant inhibition of immune cell infiltration, such as eosinophils, lymphocytes, macrophages and neutrophils in BAL fluid. Histopathological analysis also showed that Foxp3 overexpression inhibits inflammatory cell recruitment and collagen deposition in lung tissues. In addition, expression of inflammatory and fibrosis-related genes was decreased in the Foxp3 expression adenovirus-infected group. These results suggest that Foxp3 expression in lungs holds considerable therapeutic potential for attenuating inflammation and fibrosis in radiation-induced lung injury.
Full Text
https://www.nature.com/gt/journal/v24/n2/full/gt201686a.html
DOI
10.1038/gt.2016.86.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Cho, Jae Ho(조재호) ORCID logo https://orcid.org/0000-0001-9966-5157
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154523
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