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Identification of radiation response genes and proteins from mouse pulmonary tissues after high-dose per fraction irradiation of limited lung volumes

Authors
 Hee Jin  ;  Seulgi Jeon  ;  Ga-Young Kang  ;  Hae-June Lee  ;  Jaeho Cho  ;  Yun-Sil Lee 
Citation
 INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, Vol.93(2) : 184-193, 2017 
Journal Title
 INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 
ISSN
 0955-3002 
Issue Date
2017
MeSH
Animals ; Dose Hypofractionation ; Dose-Response Relationship, Radiation ; Gene Expression Regulation/radiation effects* ; Lung/metabolism* ; Lung/radiation effects* ; Male ; Mice ; Mice, Inbred C57BL ; Proteome/metabolism* ; Radiation Pneumonitis/etiology ; Radiation Pneumonitis/metabolism* ; Radiosurgery/adverse effects*
Keywords
High-dose per fraction irradiation ; microarray ; mouse lung ; protein antibody array
Abstract
PURPOSE: The molecular effects of focal exposure of limited lung volumes to high-dose per fraction irradiation (HDFR) such as stereotactic body radiotherapy (SBRT) have not been fully characterized. In this study, we used such an irradiation system and identified the genes and proteins after HDFR to mouse lung, similar to those associated with human therapy. METHODS AND MATERIALS: High focal radiation (90 Gy) was applied to a 3-mm volume of the left lung of C57BL6 mice using a small-animal stereotactic irradiator. As well as histological examination for lungs, a cDNA micro array using irradiated lung tissues and a protein array of sera were performed until 4 weeks after irradiation, and radiation-responsive genes and proteins were identified. For comparison, the long-term effects (12 months) of 20 Gy radiation wide-field dose to the left lung were also investigated. RESULTS: The genes ermap, epb4.2, cd200r3 (up regulation) and krt15, hoxc4, gdf2, cst9, cidec, and bnc1 (down-regulation) and the proteins of AIF, laminin, bNOS, HSP27, β-amyloid (upregulation), and calponin (downregulation) were identified as being responsive to 90 Gy HDFR. The gdf2, cst9, and cidec genes also responded to 20 Gy, suggesting that they are universal responsive genes in irradiated lungs. No universal proteins were identified in both 90 Gy and 20 Gy. Calponin, which was downregulated in protein antibody array analysis, showed a similar pattern in microarray data, suggesting a possible HDFR responsive serum biomarker that reflects gene alteration of irradiated lung tissue. These genes and proteins also responded to the lower doses of 20 Gy and 50 Gy HDFR. CONCLUSIONS: These results suggest that identified candidate genes and proteins are HDFR-specifically expressed in lung damage induced by HDFR relevant to SBRT in humans.
Full Text
http://www.tandfonline.com/doi/full/10.1080/09553002.2017.1235297
DOI
10.1080/09553002.2017.1235297
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Cho, Jae Ho(조재호) ORCID logo https://orcid.org/0000-0001-9966-5157
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154357
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