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Functional improvement of hemostatic dressing by addition of recombinant batroxobin

 Gyeung Mi Seon  ;  Mi Hee Lee  ;  Byeong-Ju Kwon  ;  Min Sung Kim  ;  Min-Ah Koo  ;  Dohyun Kim  ;  Young Seomun  ;  Jong-Tak Kim  ;  Jong-Chul Park 
 Acta Biomaterialia, Vol.48 : 175-185, 2017 
Journal Title
 Acta Biomaterialia 
Issue Date
Animals ; Bandages* ; Batroxobin/pharmacology* ; Blood Coagulation/drug effects ; Cattle ; Disease Models, Animal ; Femoral Artery/drug effects ; Femoral Artery/pathology ; Fibrinogen/metabolism ; Hemorrhage/pathology ; Hemostatics/pharmacology* ; Liver/drug effects ; Liver/pathology ; Microscopy, Electron, Scanning ; Nephelometry and Turbidimetry ; Platelet Activation/drug effects ; Rats, Sprague-Dawley ; Recombinant Proteins/pharmacology*
Coagulation ; Hemostasis ; Hemostatic dressings ; Natural substance ; Recombinant batroxobin
Although a number of natural materials have been used as hemostatic agents, many substances do not act quickly enough. Here, we created a novel dressings using collagen and chitosan with recombinant batroxobin (r-Bat) to promote faster and more effective hemostasis. We hypothesized that r-Bat would promote synergetic blood coagulation because it contains a blood coagulation active site different than those of collagen and chitosan. Our results suggest that each substances can maintain hemostatic properties while in the mixed dressings and that our novel hemostatic dressings promotes potent control of bleeding, as demonstrated by a whole blood assay and rat hemorrhage model. In a rat femoral artery model, the scaffold with a high r-Bat concentration more rapidly controlled excessive bleeding. This novel dressings has enormous possible for rapidly controlling bleeding and it improves upon the effect of collagen and chitosan used alone. Our novel r-Bat dressings is a possible candidate for improving preoperative care and displays promising properties as an absorbable agent in hemostasis. STATEMENT OF SIGNIFICANCE: Despite the excellent hemostatic properties of collagen and chitosan pads, they reported to brittle behavior and lack sufficient hemostatic effect within relevant time. Therefore, we created a novel pad using collagen and chitosan with recombinant batroxobin (r-Bat). r-Bat acts as a thrombin-like enzyme in the coagulation cascade. Specifically, r-Bat, in contrast to thrombin, only splits fibrinopeptide A off and does not influence other hemostatic factors or cells, which makes it clinically useful as a stable hemostatic agent. Also the materials in the pad have synergetic effect because they have different hemostatic mechanisms in the coagulation cascade. This report propose the novel hemostatic pad isreasonable that a great potential for excessive bleeding injury and improve effects of natural substance hemostatic pad.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
구민아(Koo, Min-Ah) ORCID logo https://orcid.org/0000-0002-8671-1131
권병주(Kwon, Byeong-Ju) ORCID logo https://orcid.org/0000-0001-9916-0546
박종철(Park, Jong Chul) ORCID logo https://orcid.org/0000-0003-0083-5991
선경미(Seon, Gyeung Mi)
이미희(Lee, Mi Hee) ORCID logo https://orcid.org/0000-0002-9630-7044
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