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Cytochrome P450 1B1 inhibition suppresses tumorigenicity of prostate cancer via caspase-1 activation

Authors
 Inik Chang  ;  Yozo Mitsui  ;  Seul Ki Kim  ;  Ji Su Sun  ;  Hye Sook Jeon  ;  Jung Yun Kang  ;  Nam Ju Kang  ;  Shinichiro Fukuhara  ;  Ankurpreet Gill  ;  Varahram Shahryari  ;  Z. Laura Tabatabai  ;  Kirsten L. Greene  ;  Rajvir Dahiya  ;  Dong Min Shin  ;  Yuichiro Tanaka 
Citation
 Oncotarget, Vol.8(24) : 39087-39100, 2017 
Journal Title
 Oncotarget 
Issue Date
2017
Keywords
CYP1B1 ; caspase-1 ; prostate cancer ; shRNA ; tumorigenicity
Abstract
Cytochrome P450 1B1 (CYP1B1) is recognized as a universal tumor biomarker and a feasible therapeutic target due to its specific overexpression in cancer tissues. Despite its up-regulation in prostate cancer (PCa), biological significance and clinicopathological features of CYP1B1 are still elusive. Here, we show that overexpression or hyperactivation of CYP1B1 stimulated proliferative, migratory and invasive potential of non-tumorigenic PCa cells. Attenuation of CYP1B1 with its specific small hairpin (sh) RNAs greatly reduced proliferation through apoptotic cell death and impaired migration and invasion in PCa cells. Intratumoral injection of CYP1B1 shRNA attenuated growth of pre-existing tumors. The antitumor effect of CYP1B1 shRNA was also observed in prostate tumor xenograft mouse models. Among the genes altered by CYP1B1 knockdown, reduction of caspase-1 (CASP1) activity attenuated the antitumor effect of CYP1B1 inhibition. Indeed, CYP1B1 regulates CASP1 expression or activity. Finally, CYP1B1 expression was increased in higher grades of PCa and overall survival was significantly reduced in patients with high levels of CYP1B1 protein. CYP1B1 expression was reversely associated with CASP1 expression in clinical tissue samples. Together, our results demonstrate that CYP1B1 regulates PCa tumorigenesis by inhibiting CASP1 activation. Thus, the CYP1B1-CASP1 axis may be useful as a potential biomarker and a therapeutic target for PCa.
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DOI
10.18632/oncotarget.16598.
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
신동민(Shin, Dong Min) ORCID logo https://orcid.org/0000-0001-6042-0435
장인익(Chang, In Ik)
전혜숙(Jeon, Hye Sook)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154260
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