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In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe

Authors
 Xiaoqiang Chen  ;  Dayoung Lee  ;  Sungsook Yu  ;  Gyoungmi Kim  ;  Songyi Lee  ;  Yejin Cho  ;  Haengdueng Jeong  ;  Ki Taek Nam  ;  Juyoung Yoon 
Citation
 BIOMATERIALS, Vol.122 : 130-140, 2017 
Journal Title
 BIOMATERIALS 
ISSN
 0142-9612 
Issue Date
2017
MeSH
Animals ; Cathepsin B ; Cell Line, Tumor ; Fluorescent Dyes/chemical synthesis ; Humans ; Infrared Rays ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Microscopy, Fluorescence/methods* ; Neoplasms, Experimental/drug therapy* ; Neoplasms, Experimental/metabolism ; Neoplasms, Experimental/pathology* ; Oligopeptides/chemistry ; Oligopeptides/pharmacokinetics* ; Photochemotherapy/methods* ; Photosensitizing Agents/administration & dosage ; Theranostic Nanomedicine/methods* ; Treatment Outcome
Keywords
Enzyme-activated fluorescence ; Multiple functional probe ; NIR bioimaging ; Specific phototherapy
Abstract
The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe (CyA-P-CyB) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation.
Full Text
http://www.sciencedirect.com/science/article/pii/S0142961217300285
DOI
10.1016/j.biomaterials.2017.01.020.
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Nam, Ki Taek(남기택)
Yu, Sungsook(유성숙)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154190
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