0 373

Cited 3 times in

Identification of essential genes of Pseudomonas aeruginosa for its growth in airway mucus

Authors
 Mohammed Abd Alrahman  ;  Sang Sun Yoon 
Citation
 JOURNAL OF MICROBIOLOGY, Vol.55(1) : 68-74, 2017 
Journal Title
JOURNAL OF MICROBIOLOGY
ISSN
 1225-8873 
Issue Date
2017
MeSH
Bacterial Proteins/genetics* ; Culture Media ; Cystic Fibrosis/microbiology ; DNA Transposable Elements ; Drug Discovery ; Gene Expression Profiling ; Gene Library ; Genes, Essential ; Mucins/metabolism* ; Mucus/microbiology* ; Mutation ; Peptide Hydrolases/genetics* ; Pseudomonas Infections/microbiology ; Pseudomonas ; aeruginosa/genetics* ; Pseudomonas aeruginosa/growth & development* ; Pseudomonas aeruginosa/pathogenicity ; Pseudomonas ; aeruginosa/physiology ; Type II Secretion Systems/genetics* ; Virulence/genetics
Keywords
Pseudomonas aeruginosa ; airway infection ; mucin ; protease ; type II secretion system
Abstract
Pseudomonas aeruginosa has been identified as an important causative agent of airway infection, mainly in cystic fibrosis. This disease is characterized by defective mucociliary clearance induced in part by mucus hyper-production. Mucin is a major component of airway mucus and is heavily O-glycosylated, with a protein backbone. Airway infection is known to be established with bacterial adhesion to mucin. However, the genes involved in mucin degradation or utilization remain elusive. In this study, we sought to provide a genetic basis of P. aeruginosa airway growth by identifying those genes. First, using RNASeq analyses, we compared genome-wide expression profiles of PAO1, a prototype P. aeruginosa laboratory strain, grown in M9-mucin (M9M) and M9-glucose (M9G) media. Additionally, a PAO1 transposon (Tn) insertion mutants library was screened for mutants defective in growth in M9M medium. One mutant with a Tn insertion in the xcpU gene (PA3100) was determined to exhibit faulty growth in M9M medium. This gene contributes to the type II secretion system, suggesting that P. aeruginosa uses this secretion system to produce a number of proteins to break down and assimilate the mucin molecule. Furthermore, we screened the PAO1 genome for genes with protease activity. Of 13 mutants, one with mutation in PA3247 gene exhibited defective growth in M9M, suggesting that the PA3247-encoded protease plays a role in mucin utilization. Further mechanistic dissection of this particular process will reveal new drug targets, the inhibition of which could control recalcitrant P. aeruginosa infections.
Full Text
https://link.springer.com/article/10.1007%2Fs12275-017-6515-3
DOI
10.1007/s12275-017-6515-3
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154139
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links