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Relaxin Modulates the Expression of MMPs and TIMPs in Fibroblasts of Patients with Carpal Tunnel Syndrome

Authors
 Young-Mi Kang  ;  Hwan-Mo Lee  ;  Seong-Hwan Moon  ;  Ho Kang  ;  Yun-Rak Choi 
Citation
 YONSEI MEDICAL JOURNAL, Vol.58(2) : 415-422, 2017 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
2017
MeSH
Carpal Tunnel Syndrome/metabolism* ; Cells, Cultured ; Collagen Type I/metabolism ; Collagen Type III/metabolism ; Collagen Type IV/metabolism ; Extracellular Matrix/metabolism ; Fibroblasts/drug effects ; Fibroblasts/metabolism* ; Fibronectins/metabolism ; Humans ; Matrix Metalloproteinases/metabolism* ; Relaxin/pharmacology* ; Smad2 Protein/metabolism ; Tissue Inhibitor of Metalloproteinases/metabolism* ; Transforming Growth Factor beta/metabolism ; Transforming Growth Factor beta1/pharmacology
Keywords
Carpal tunnel syndrome ; collagens ; matrix metalloproteinases ; relaxin ; subsynovial connective tissue
Abstract
PURPOSE: The aim of this study was to investigate the anti-fibrotic effect of relaxin in subsynovial fibroblasts activated by transforming growth factor beta (TGF-β). MATERIALS AND METHODS: To test the anti-fibrotic effect of an adenovirus-relaxin construct (Ad-RLN) on subsynovial fibroblasts in vitro, cells from subsynovial connective tissue of patients with carpal tunnel syndrome were activated with TGF-β1 and exposed to Ad-RLN (as a therapeutic gene) or adenovirus-lacZ construct (as a marker gene) for four hours. Subsynovial fibroblast cultures without adenoviral exposure served as controls. RESULTS: We observed induction of gene expressions of collagen I, III and IV, as well as the abatement of alpha-smooth muscle actin (a-SMA) synthesis, Smad2 phosphorylation, and fibronectin at the protein level, in comparison to controls. In addition, protein expressions of matrix metalloproteinase (MMP) I was significantly induced, whereas the protein expressions of tissue inhibitor of metalloproteinases (TIMP) I and IV were reduced due to relaxin expression. CONCLUSION: RLN prevents excessive synthesis of extracellular matrix by reducing the expressions of its components, such as fibronectin, a-SMA, and phosphorylated Smad2, by increasing the expression of MMPs; and by decreasing the expression of TIMPs.
Files in This Item:
T201700214.pdf Download
DOI
10.3349/ymj.2017.58.2.415
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Young Mi(강영미) ORCID logo https://orcid.org/0000-0002-7686-6316
Kang, Ho(강호)
Moon, Seong Hwan(문성환)
Lee, Hwan Mo(이환모) ORCID logo https://orcid.org/0000-0002-5405-3832
Choi, Yun Rak(최윤락)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154132
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