Cited 9 times in
Relaxin Modulates the Expression of MMPs and TIMPs in Fibroblasts of Patients with Carpal Tunnel Syndrome
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 문성환 | - |
dc.contributor.author | 이환모 | - |
dc.contributor.author | 최윤락 | - |
dc.contributor.author | 강호 | - |
dc.contributor.author | 강영미 | - |
dc.date.accessioned | 2017-11-02T08:08:49Z | - |
dc.date.available | 2017-11-02T08:08:49Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154132 | - |
dc.description.abstract | PURPOSE: The aim of this study was to investigate the anti-fibrotic effect of relaxin in subsynovial fibroblasts activated by transforming growth factor beta (TGF-β). MATERIALS AND METHODS: To test the anti-fibrotic effect of an adenovirus-relaxin construct (Ad-RLN) on subsynovial fibroblasts in vitro, cells from subsynovial connective tissue of patients with carpal tunnel syndrome were activated with TGF-β1 and exposed to Ad-RLN (as a therapeutic gene) or adenovirus-lacZ construct (as a marker gene) for four hours. Subsynovial fibroblast cultures without adenoviral exposure served as controls. RESULTS: We observed induction of gene expressions of collagen I, III and IV, as well as the abatement of alpha-smooth muscle actin (a-SMA) synthesis, Smad2 phosphorylation, and fibronectin at the protein level, in comparison to controls. In addition, protein expressions of matrix metalloproteinase (MMP) I was significantly induced, whereas the protein expressions of tissue inhibitor of metalloproteinases (TIMP) I and IV were reduced due to relaxin expression. CONCLUSION: RLN prevents excessive synthesis of extracellular matrix by reducing the expressions of its components, such as fibronectin, a-SMA, and phosphorylated Smad2, by increasing the expression of MMPs; and by decreasing the expression of TIMPs. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Carpal Tunnel Syndrome/metabolism* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Collagen Type I/metabolism | - |
dc.subject.MESH | Collagen Type III/metabolism | - |
dc.subject.MESH | Collagen Type IV/metabolism | - |
dc.subject.MESH | Extracellular Matrix/metabolism | - |
dc.subject.MESH | Fibroblasts/drug effects | - |
dc.subject.MESH | Fibroblasts/metabolism* | - |
dc.subject.MESH | Fibronectins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Matrix Metalloproteinases/metabolism* | - |
dc.subject.MESH | Relaxin/pharmacology* | - |
dc.subject.MESH | Smad2 Protein/metabolism | - |
dc.subject.MESH | Tissue Inhibitor of Metalloproteinases/metabolism* | - |
dc.subject.MESH | Transforming Growth Factor beta/metabolism | - |
dc.subject.MESH | Transforming Growth Factor beta1/pharmacology | - |
dc.title | Relaxin Modulates the Expression of MMPs and TIMPs in Fibroblasts of Patients with Carpal Tunnel Syndrome | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Orthopedic Surgery | - |
dc.contributor.googleauthor | Young-Mi Kang | - |
dc.contributor.googleauthor | Hwan-Mo Lee | - |
dc.contributor.googleauthor | Seong-Hwan Moon | - |
dc.contributor.googleauthor | Ho Kang | - |
dc.contributor.googleauthor | Yun-Rak Choi | - |
dc.identifier.doi | 10.3349/ymj.2017.58.2.415 | - |
dc.contributor.localId | A03333 | - |
dc.contributor.localId | A04136 | - |
dc.contributor.localId | A05082 | - |
dc.contributor.localId | A01365 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 28120574 | - |
dc.subject.keyword | Carpal tunnel syndrome | - |
dc.subject.keyword | collagens | - |
dc.subject.keyword | matrix metalloproteinases | - |
dc.subject.keyword | relaxin | - |
dc.subject.keyword | subsynovial connective tissue | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.alternativeName | Lee, Hwan Mo | - |
dc.contributor.alternativeName | Choi, Yun Rak | - |
dc.contributor.alternativeName | Kang, Ho | - |
dc.contributor.affiliatedAuthor | Lee, Hwan Mo | - |
dc.contributor.affiliatedAuthor | Choi, Yun Rak | - |
dc.contributor.affiliatedAuthor | Kang, Ho | - |
dc.contributor.affiliatedAuthor | Moon, Seong Hwan | - |
dc.citation.title | Yonsei Medical Journal | - |
dc.citation.volume | 58 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 415 | - |
dc.citation.endPage | 422 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.58(2) : 415-422, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 41631 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.