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Cell culture-adaptive mutations of NS5A affect replication of hepatitis C virus differentially depending on the viral genotypes

Authors
 Aeri Chung  ;  Bora Jin  ;  Kwang-Hyub Han  ;  Sang Hoon Ahn  ;  Seungtaek Kim 
Citation
 JOURNAL OF MEDICAL VIROLOGY, Vol.89(1) : 146-152, 2017 
Journal Title
 JOURNAL OF MEDICAL VIROLOGY 
ISSN
 0146-6615 
Issue Date
2017
MeSH
Adaptation, Biological ; Cell Line ; DNA Mutational Analysis ; Genotype* ; Hepacivirus/genetics ; Hepacivirus/physiology* ; Hepatocytes/virology ; Humans ; Mutant Proteins/genetics* ; Viral Nonstructural ; Proteins/genetics* ; Virus Cultivation ; Virus Replication*
Keywords
NS5A ; cell culture-adaptive mutation ; genotype ; hepatitis C virus
Abstract
Most of HCV RNAs require cell culture-adaptive mutations for efficient replication in cell culture and a number of such mutations have been described including a well-known S2204I substitution mutation in NS5A protein. In contrast, the replication of genotype 2a JFH1 RNA in cell culture does not require any cell culture-adaptive mutation. Rather, the presence of S2204I mutation impaired the JFH1 RNA replication. In this study, we examined the effect of reversions and substitutions of NS5A cell culture-adaptive mutations on virus replication in different genotypic backgrounds after either placing genotype 1a NS5A in the genotype 2a JFH1 or vice versa. The results from this investigation suggest that the S2204I mutation affects HCV RNA replication differentially depending on the viral genotypes but that the effect was not simply explained by the genotypic background. Perhaps, the effect of the S2204I mutation on HCV replication reflects both intra- and intergenic interactions of NS5A protein. J. Med. Virol. 89:146-152, 2017. © 2016 Wiley Periodicals, Inc.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jmv.24591/abstract
DOI
10.1002/jmv.24591
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seung Taek(김승택)
Ahn, Sang Hoon(안상훈) ORCID logo https://orcid.org/0000-0002-3629-4624
Han, Kwang-Hyub(한광협) ORCID logo https://orcid.org/0000-0003-3960-6539
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154102
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