Cited 3 times in
Cell culture-adaptive mutations of NS5A affect replication of hepatitis C virus differentially depending on the viral genotypes
DC Field | Value | Language |
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dc.contributor.author | 김승택 | - |
dc.contributor.author | 안상훈 | - |
dc.contributor.author | 한광협 | - |
dc.date.accessioned | 2017-11-02T08:07:16Z | - |
dc.date.available | 2017-11-02T08:07:16Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0146-6615 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/154102 | - |
dc.description.abstract | Most of HCV RNAs require cell culture-adaptive mutations for efficient replication in cell culture and a number of such mutations have been described including a well-known S2204I substitution mutation in NS5A protein. In contrast, the replication of genotype 2a JFH1 RNA in cell culture does not require any cell culture-adaptive mutation. Rather, the presence of S2204I mutation impaired the JFH1 RNA replication. In this study, we examined the effect of reversions and substitutions of NS5A cell culture-adaptive mutations on virus replication in different genotypic backgrounds after either placing genotype 1a NS5A in the genotype 2a JFH1 or vice versa. The results from this investigation suggest that the S2204I mutation affects HCV RNA replication differentially depending on the viral genotypes but that the effect was not simply explained by the genotypic background. Perhaps, the effect of the S2204I mutation on HCV replication reflects both intra- and intergenic interactions of NS5A protein. J. Med. Virol. 89:146-152, 2017. © 2016 Wiley Periodicals, Inc. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Wiley-Liss | - |
dc.relation.isPartOf | JOURNAL OF MEDICAL VIROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adaptation, Biological | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | DNA Mutational Analysis | - |
dc.subject.MESH | Genotype* | - |
dc.subject.MESH | Hepacivirus/genetics | - |
dc.subject.MESH | Hepacivirus/physiology* | - |
dc.subject.MESH | Hepatocytes/virology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mutant Proteins/genetics* | - |
dc.subject.MESH | Viral Nonstructural | - |
dc.subject.MESH | Proteins/genetics* | - |
dc.subject.MESH | Virus Cultivation | - |
dc.subject.MESH | Virus Replication* | - |
dc.title | Cell culture-adaptive mutations of NS5A affect replication of hepatitis C virus differentially depending on the viral genotypes | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Life Science | - |
dc.contributor.googleauthor | Aeri Chung | - |
dc.contributor.googleauthor | Bora Jin | - |
dc.contributor.googleauthor | Kwang-Hyub Han | - |
dc.contributor.googleauthor | Sang Hoon Ahn | - |
dc.contributor.googleauthor | Seungtaek Kim | - |
dc.identifier.doi | 10.1002/jmv.24591 | - |
dc.contributor.localId | A02226 | - |
dc.contributor.localId | A04268 | - |
dc.contributor.localId | A00661 | - |
dc.relation.journalcode | J01587 | - |
dc.identifier.eissn | 1096-9071 | - |
dc.identifier.pmid | 27253731 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jmv.24591/abstract | - |
dc.subject.keyword | NS5A | - |
dc.subject.keyword | cell culture-adaptive mutation | - |
dc.subject.keyword | genotype | - |
dc.subject.keyword | hepatitis C virus | - |
dc.contributor.alternativeName | Kim, Seung Taek | - |
dc.contributor.alternativeName | Ahn, Sang Hoon | - |
dc.contributor.alternativeName | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Ahn, Sang Hoon | - |
dc.contributor.affiliatedAuthor | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Kim, Seung Taek | - |
dc.citation.title | Journal of Medical Virology | - |
dc.citation.volume | 89 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 146 | - |
dc.citation.endPage | 152 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MEDICAL VIROLOGY, Vol.89(1) : 146-152, 2017 | - |
dc.date.modified | 2017-11-01 | - |
dc.identifier.rimsid | 41598 | - |
dc.type.rims | ART | - |
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