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Loss of Notch3 Signaling Enhances Osteogenesis of Mesenchymal Stem Cells from Mandibular Torus

Authors
 X.W. Dou  ;  W. Park  ;  S. Lee  ;  Q.Z. Zhang  ;  L.R. Carrasco  ;  A.D. Le 
Citation
 JOURNAL OF DENTAL RESEARCH, Vol.96(3) : 347-354, 2017 
Journal Title
JOURNAL OF DENTAL RESEARCH
ISSN
 0022-0345 
Issue Date
2017
MeSH
Aged ; Blotting, Western ; Cell Differentiation ; Exostoses/pathology* ; Female ; Humans ; Male ; Mandible/abnormalities* ; Mesenchymal Stromal Cells/pathology* ; Middle Aged ; Osteogenesis/physiology* ; RNA, Small Interfering ; Real-Time Polymerase Chain Reaction ; Receptor, Notch3/metabolism* ; Signal Transduction ; Transfection
Keywords
adult stem cell(s) ; bone formation ; cell signaling ; gene expression ; jaw bone anomalies ; oral and maxillofacial surgery
Abstract
Mandibular torus (MT) is a common intraoral osseous outgrowth located on the lingual surface of the mandible. Histologic features include hyperplastic bone consisting of mature cortical and trabecular bone. Some theories on the etiology of MT have been postulated, such as genetic factors, masticatory hyperfunction, trauma, and continued growth, but the underlying mechanism remains largely unknown. In this study, we investigated the potential role of mesenchymal stem cells (MSCs) derived from human MT in the pathogenesis of bone outgrowth. We demonstrated that MT harbored a distinct subpopulation of MSCs, with enhanced osteogenic and decreased adipogenic differentiation capacities, as compared with their counterparts from normal jaw bone. The increased osteogenic differentiation of mandibular torus MSCs was associated with the suppression of Notch3 signaling and its downstream target genes, Jag1 and Hey1, and a reciprocal increase in the transcriptional activation of ATF4 and NFATc1 genes. Targeted knockdown of Notch3 expression by transient siRNA transfection promoted the expression of osteogenic transcription factors in normal jaw bone MSCs. Our data suggest that the loss of Notch3 signaling may contribute partly to bone outgrowth in MT, as mediated by enhanced MSC-driven osteogenic differentiation in the jaw bone.
Full Text
http://journals.sagepub.com/doi/abs/10.1177/0022034516680349
DOI
10.1177/0022034516680349
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과) > 1. Journal Papers
Yonsei Authors
Park, Wonse(박원서) ORCID logo https://orcid.org/0000-0002-2081-1156
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154092
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