0 325

Cited 0 times in

Defective autophagy activity and its association with spinal damage in patients with ankylosing spondylitis

 Min-Chan Park  ;  Hye Won Kim  ;  Sang-Won Lee  ;  Jason Jungsik Song  ;  Yong-Beom Park 
 JOINT BONE SPINE, Vol.84(5) : 583-587, 2017 
Journal Title
Issue Date
Ankylosing spondylitis ; Autophagy ; Cytokine ; Disease activity ; Spinal damage
OBJECTIVES: This study was performed to determine the expression levels of autophagy-related genes in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and to investigate their associations with clinical parameters reflecting disease activity and radiographic progression and with cytokine levels of AS patients. METHODS: PBMCs from 53 AS patients and 49 healthy controls were obtained and mRNA expression levels of LC3, belcin1, and ATG5 were determined using quantitative real-time PCR. Ankylosing Spondylitis Disease Activity Score (ASDAS)-CRP and modified Stoke AS Spinal Score (mSASSS) were assessed at the time of blood sampling. Serum concentrations of TNF-α, IL-17, and IL-23 were determined in the serum of AS patients using enzyme-linked immunosorbent assay. RESULTS: LC3, beclin1, and ATG5 mRNAs were constitutively expressed in PBMCs of AS patients and healthy controls; however, expression of all three genes was significantly decreased in PBMCs of AS patients compared with those from controls. Expression levels of the autophagy-related genes were not significantly correlated with ASDAS-CRP or serum TNF-α, IL-17, and IL-23 concentrations. However, LC3 and beclin1 mRNA levels showed significant negative correlations with mSASSS of AS patients (r=-0.805, P<0.01 for LC3 and r=-0.712, P<0.01 for beclin1). CONCLUSION: AS patients have decreased autophagy-related gene expressions and AS patients with more advanced spinal damage have further decreased LC3 and beclin1 expression levels. These results suggest that AS patients have defective autophagy activity and that compromised autophagy may contribute to the progression of spinal damage in AS.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Min Chan(박민찬) ORCID logo https://orcid.org/0000-0003-1189-7637
Park, Yong Beom(박용범)
Song, Jason Jungsik(송정식) ORCID logo https://orcid.org/0000-0003-0662-7704
Lee, Sang-Won(이상원) ORCID logo https://orcid.org/0000-0002-8038-3341
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.