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Defective autophagy activity and its association with spinal damage in patients with ankylosing spondylitis

Authors
 Min-Chan Park  ;  Hye Won Kim  ;  Sang-Won Lee  ;  Jason Jungsik Song  ;  Yong-Beom Park 
Citation
 Joint Bone Spine, Vol.84(5) : 583-587, 2017 
Journal Title
 Joint Bone Spine 
ISSN
 1297-319X 
Issue Date
2017
Keywords
Ankylosing spondylitis ; Autophagy ; Cytokine ; Disease activity ; Spinal damage
Abstract
OBJECTIVES: This study was performed to determine the expression levels of autophagy-related genes in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and to investigate their associations with clinical parameters reflecting disease activity and radiographic progression and with cytokine levels of AS patients. METHODS: PBMCs from 53 AS patients and 49 healthy controls were obtained and mRNA expression levels of LC3, belcin1, and ATG5 were determined using quantitative real-time PCR. Ankylosing Spondylitis Disease Activity Score (ASDAS)-CRP and modified Stoke AS Spinal Score (mSASSS) were assessed at the time of blood sampling. Serum concentrations of TNF-α, IL-17, and IL-23 were determined in the serum of AS patients using enzyme-linked immunosorbent assay. RESULTS: LC3, beclin1, and ATG5 mRNAs were constitutively expressed in PBMCs of AS patients and healthy controls; however, expression of all three genes was significantly decreased in PBMCs of AS patients compared with those from controls. Expression levels of the autophagy-related genes were not significantly correlated with ASDAS-CRP or serum TNF-α, IL-17, and IL-23 concentrations. However, LC3 and beclin1 mRNA levels showed significant negative correlations with mSASSS of AS patients (r=-0.805, P<0.01 for LC3 and r=-0.712, P<0.01 for beclin1). CONCLUSION: AS patients have decreased autophagy-related gene expressions and AS patients with more advanced spinal damage have further decreased LC3 and beclin1 expression levels. These results suggest that AS patients have defective autophagy activity and that compromised autophagy may contribute to the progression of spinal damage in AS.
Full Text
http://www.sciencedirect.com/science/article/pii/S1297319X16301609
DOI
10.1016/j.jbspin.2016.09.005
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
박민찬(Park, Min Chan) ORCID logo https://orcid.org/0000-0003-1189-7637
박용범(Park, Yong Beom)
송정식(Song, Jungsik Jason) ORCID logo https://orcid.org/0000-0003-0662-7704
이상원(Lee, Sang Won) ORCID logo https://orcid.org/0000-0002-8038-3341
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154066
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