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Anti-helminthic niclosamide inhibits Ras-driven oncogenic transformation via activation of GSK-3

 Sung Yong Ahn  ;  Ji Hye Yang  ;  Nam Hee Kim  ;  Kyungro Lee  ;  Yong Hoon Cha  ;  Jun Seop Yun  ;  Hee Eun Kang  ;  Yoonmi Lee  ;  Jiwon Choi  ;  Hyun Sil Kim  ;  Jong In Yook 
 ONCOTARGET , Vol.8(19) : 31856-31863, 2017 
Journal Title
Issue Date
Animals ; Cell Line, Tumor ; Cell Transformation, Neoplastic/drug effects* ; Cell Transformation, Neoplastic/genetics* ; Cell Transformation, Neoplastic/metabolism* ; Disease Models, Animal ; Enzyme Activation/drug effects ; Genes, ras* ; Glycogen Synthase Kinase 3/metabolism* ; Humans ; Models, Biological ; Mutation ; Niclosamide/pharmacology* ; Xenograft Model Antitumor Assays
GSK-3 ; Ras oncogene ; epithelial-mesenchymal transition (EMT) ; niclosamide
Despite the importance of Ras oncogenes as a therapeutic target in human cancer, their 'undruggable' tertiary structures limit the effectiveness of anti-Ras drugs. Canonical Wnt signaling contributes to Ras activity by glycogen synthase kinase 3 (GSK-3)-dependent phosphorylation at the C-terminus and subsequent degradation. In the accompanying report, we show that the anti-helminthic niclosamide directly binds to GSK-3 and inhibits Axin functions in colon cancer cells, with reversion of Snail-mediated epithelial-mesenchymal transition. In this study, we report that niclosamide effectively suppresses Ras and nuclear NFAT activities regardless of the mutational status of Ras at nM levels. Mechanistically, niclosamide increased endogenous GSK-3 activity, shortening the half-life of mutant Ras. Further, niclosamide activates Raf-1 kinase inhibitory protein, a downstream target of Snail repressor. Niclosamide treatment attenuates Ras-induced oncogenic potential in vitro and in vivo. These findings provide a clinically available repositioned Ras inhibitor as well as a novel strategy for inhibiting the Ras via GSK-3.
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2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Nam Hee(김남희) ORCID logo https://orcid.org/0000-0002-3087-5276
Kim, Hyun Sil(김현실) ORCID logo https://orcid.org/0000-0003-3614-1764
Ahn, Sung Yong(안성용) ORCID logo https://orcid.org/0000-0002-6029-1853
Yang, Ji Hye(양지혜)
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
Lee, Yoon Mi(이윤미)
Cha, Yong Hoon(차용훈) ORCID logo https://orcid.org/0000-0003-1761-3260
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