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Clonal Dissemination of Pseudomonas aeruginosa Sequence Type 235 Isolates Carrying blaIMP-6 and Emergence of blaGES-24 and blaIMP-10 on Novel Genomic Islands PAGI-15 and -16 in South Korea

 Jun Sung Hong  ;  Eun-Jeong Yoon  ;  Hyukmin Lee  ;  Seok Hoon Jeong  ;  Kyungwon Lee 
 Antimicrobial Agents and Chemotherapy, Vol.60(12) : 7216-7223, 2016 
Journal Title
 Antimicrobial Agents and Chemotherapy 
Issue Date
Anti-Bacterial Agents/pharmacology* ; Bacterial Proteins/genetics* ; Carbapenems/pharmacology* ; DNA, Bacterial/genetics ; Electrophoresis, Gel, Pulsed-Field ; Genomic Islands/genetics ; Humans ; Integrons/genetics ; Microbial Sensitivity Tests ; Molecular Epidemiology ; Multilocus Sequence Typing ; Polymerase Chain Reaction ; Pseudomonas Infections/drug therapy ; Pseudomonas Infections/microbiology ; Pseudomonas aeruginosa/classification ; Pseudomonas aeruginosa/drug effects* ; Pseudomonas aeruginosa/genetics* ; Pseudomonas aeruginosa/isolation & purification ; Republic of Korea ; beta-Lactamases/genetics*
A total of 431 Pseudomonas aeruginosa clinical isolates were collected from 29 general hospitals in South Korea in 2015. Antimicrobial susceptibility was tested by the disk diffusion method, and MICs of carbapenems were determined by the agar dilution method. Carbapenemase genes were amplified by PCR and sequenced, and the structures of class 1 integrons surrounding the carbapenemase gene cassettes were analyzed by PCR mapping. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed for strain typing. Whole-genome sequencing was carried out to analyze P. aeruginosa genomic islands (PAGIs) carrying the blaIMP-6, blaIMP-10, and blaGES-24 genes. The rates of carbapenem-nonsusceptible and carbapenemase-producing P. aeruginosa isolates were 34.3% (148/431) and 9.5% (41/431), respectively. IMP-6 was the most prevalent carbapenemase type, followed by VIM-2, IMP-10, and GES-24. All carbapenemase genes were located on class 1 integrons of 6 different types on the chromosome. All isolates harboring carbapenemase genes exhibited genetic relatedness by PFGE (similarity > 80%); moreover, all isolates were identified as sequence type 235 (ST235), with the exception of two ST244 isolates by MLST. The blaIMP-6, blaIMP-10, and blaGES-24 genes were found to be located on two novel PAGIs, designated PAGI-15 and PAGI-16. Our data support the clonal spread of an IMP-6-producing P. aeruginosa ST235 strain, and the emergence of IMP-10 and GES-24 demonstrates the diversification of carbapenemases in P. aeruginosa in Korea.
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Yonsei Authors
이경원(Lee, Kyungwon) ORCID logo https://orcid.org/0000-0003-3788-2134
이혁민(Lee, Hyuk Min) ORCID logo https://orcid.org/0000-0002-8523-4126
정석훈(Jeong, Seok Hoon) ORCID logo https://orcid.org/0000-0001-9290-897X
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