Cited 51 times in
Clonal Dissemination of Pseudomonas aeruginosa Sequence Type 235 Isolates Carrying blaIMP-6 and Emergence of blaGES-24 and blaIMP-10 on Novel Genomic Islands PAGI-15 and -16 in South Korea
DC Field | Value | Language |
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dc.contributor.author | 이경원 | - |
dc.contributor.author | 이혁민 | - |
dc.contributor.author | 정석훈 | - |
dc.contributor.author | 홍준성 | - |
dc.date.accessioned | 2017-10-26T08:01:22Z | - |
dc.date.available | 2017-10-26T08:01:22Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0066-4804 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/152834 | - |
dc.description.abstract | A total of 431 Pseudomonas aeruginosa clinical isolates were collected from 29 general hospitals in South Korea in 2015. Antimicrobial susceptibility was tested by the disk diffusion method, and MICs of carbapenems were determined by the agar dilution method. Carbapenemase genes were amplified by PCR and sequenced, and the structures of class 1 integrons surrounding the carbapenemase gene cassettes were analyzed by PCR mapping. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were performed for strain typing. Whole-genome sequencing was carried out to analyze P. aeruginosa genomic islands (PAGIs) carrying the blaIMP-6, blaIMP-10, and blaGES-24 genes. The rates of carbapenem-nonsusceptible and carbapenemase-producing P. aeruginosa isolates were 34.3% (148/431) and 9.5% (41/431), respectively. IMP-6 was the most prevalent carbapenemase type, followed by VIM-2, IMP-10, and GES-24. All carbapenemase genes were located on class 1 integrons of 6 different types on the chromosome. All isolates harboring carbapenemase genes exhibited genetic relatedness by PFGE (similarity > 80%); moreover, all isolates were identified as sequence type 235 (ST235), with the exception of two ST244 isolates by MLST. The blaIMP-6, blaIMP-10, and blaGES-24 genes were found to be located on two novel PAGIs, designated PAGI-15 and PAGI-16. Our data support the clonal spread of an IMP-6-producing P. aeruginosa ST235 strain, and the emergence of IMP-10 and GES-24 demonstrates the diversification of carbapenemases in P. aeruginosa in Korea. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/octet-stream | - |
dc.publisher | American Society for Microbiology | - |
dc.relation.isPartOf | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anti-Bacterial Agents/pharmacology* | - |
dc.subject.MESH | Bacterial Proteins/genetics* | - |
dc.subject.MESH | Carbapenems/pharmacology* | - |
dc.subject.MESH | DNA, Bacterial/genetics | - |
dc.subject.MESH | Electrophoresis, Gel, Pulsed-Field | - |
dc.subject.MESH | Genomic Islands/genetics | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Integrons/genetics | - |
dc.subject.MESH | Microbial Sensitivity Tests | - |
dc.subject.MESH | Molecular Epidemiology | - |
dc.subject.MESH | Multilocus Sequence Typing | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Pseudomonas Infections/drug therapy | - |
dc.subject.MESH | Pseudomonas Infections/microbiology | - |
dc.subject.MESH | Pseudomonas aeruginosa/classification | - |
dc.subject.MESH | Pseudomonas aeruginosa/drug effects* | - |
dc.subject.MESH | Pseudomonas aeruginosa/genetics* | - |
dc.subject.MESH | Pseudomonas aeruginosa/isolation & purification | - |
dc.subject.MESH | Republic of Korea | - |
dc.subject.MESH | beta-Lactamases/genetics* | - |
dc.title | Clonal Dissemination of Pseudomonas aeruginosa Sequence Type 235 Isolates Carrying blaIMP-6 and Emergence of blaGES-24 and blaIMP-10 on Novel Genomic Islands PAGI-15 and -16 in South Korea | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Jun Sung Hong | - |
dc.contributor.googleauthor | Eun-Jeong Yoon | - |
dc.contributor.googleauthor | Hyukmin Lee | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.identifier.doi | 10.1128/AAC.01601-16 | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A03286 | - |
dc.contributor.localId | A03619 | - |
dc.relation.journalcode | J00189 | - |
dc.identifier.eissn | 1098-6596 | - |
dc.identifier.pmid | 27671068 | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Lee, Hyuk Min | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Lee, Hyuk Min | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.citation.volume | 60 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 7216 | - |
dc.citation.endPage | 7223 | - |
dc.identifier.bibliographicCitation | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol.60(12) : 7216-7223, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 40464 | - |
dc.type.rims | ART | - |
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