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CpG Oligodeoxynucleotide Inhibits Cockroach-Induced Asthma via Induction of IFN-γ? Th1 Cells or Foxp3? Regulatory T Cells in the Lung

Authors
 Do-Hyun Kim  ;  Jung-Ho Sohn  ;  Hong-Jai Park  ;  Jae-Hyun Lee  ;  Jung-Won Park  ;  Je-Min Choi 
Citation
 Allergy Asthma & Immunology Research, Vol.8(3) : 264-275, 2016 
Journal Title
 Allergy Asthma & Immunology Research 
ISSN
 2092-7355 
Issue Date
2016
Keywords
CpG-ODN ; Th2 inflammation ; cockroach allergen ; immune modulation
Abstract
PURPOSE: CpG oligodeoxynucleotide (CpG-ODN), a TLR9 agonist, activates innate immunity and induces Th1 response. Although the immune modulatory effect of CpG-ODN has been extensively studied, its function in cockroach extract-induced allergic asthma has not been studied. Here, we investigated the inhibitory function of CpG-ODN in cockroach extract-induced asthma in mice with different treatment schemes. METHODS: Scheme 1: BALB/C mice were intra-nasally co-administered by cockroach extract and CpG-ODN twice a week for 3 weeks; Scheme 2: The mice were intra-nasally pre-treated with CpG-ODN at day 0 and cockroach allergen challenge was performed from day 3 as in scheme 1. Scheme 3: Cockroach allergen challenge was performed as in scheme 1 and CpG-ODN was post-treated at day 21. Then, BAL cell count, flow cytometric analysis of alveolar macrophages, regulatory T cells, and lung tissue histology, Th1 and Th2 cytokines, serum IgE, cockroach specific IgE, IgG1/IgG2a ratio, and airway hyper-responsiveness were evaluated. RESULTS: Mice with repeated intra-nasal exposure to CpG-ODN showed a dramatic decrease in eosinophilic inflammation, goblet cell hyperplasia, and airway hyper-responsiveness with reduction of IL-13, IL-5, and serum IgE, cockroach specific IgE and IgG1/IgG2a ratio. This inhibitory function might be related to the up-regulation of IL-10 and CD4?Foxp3? regulatory T cells in the lung. Interestingly, one-time challenge of CpG-ODN either prior or posterior to cockroach extract exposure could modulate airway inflammation and hyper-responsiveness via increase of Th1 response. CONCLUSIONS: Collectively, our data suggest that CpG-ODN treatment modulates Th2 inflammation in the lung by induction of regulatory T cells or Th1 response in a cockroach-induced asthma model.
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DOI
10.4168/aair.2016.8.3.264
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
박중원(Park, Jung Won) ORCID logo https://orcid.org/0000-0003-0249-8749
이재현(Lee, Jae Hyun) ORCID logo https://orcid.org/0000-0002-0760-0071
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152312
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