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Long-Term Effects of Bone Marrow-Derived Mesenchymal Stem Cells in Dextran Sulfate Sodium-Induced Murine Chronic Colitis

 Hyun Jung Lee  ;  Sun-Hee Oh  ;  Hui Won Jang  ;  Ji-Hee Kwon  ;  Kyoung Jin Lee  ;  Chung Hee Kim  ;  Soo Jung Park  ;  Sung Pil Hong  ;  Jae Hee Cheon  ;  Tae Il Kim  ;  Won Ho Kim 
 GUT AND LIVER, Vol.10(3) : 412-419, 2016 
Journal Title
Issue Date
Animals ; Chronic Disease ; Colitis/chemically induced ; Colitis/prevention & control* ; Cytokines/metabolism ; Dextran Sulfate/toxicity ; Disease Models, Animal ; Female ; Irritants/toxicity ; Mesenchymal Stem Cell Transplantation* ; Mesenchymal Stromal Cells/physiology ; Mice ; Mice, Inbred C57BL
Bone marrow ; Chronic colitis ; Dextran sulfate sodium ; Inflammatory bowel disease ; Mesenchymal stem cell
BACKGROUND/AIMS: Bone marrow-derived mesenchymal stem cells (BM-MSCs) have shown beneficial effects in experimental colitis models, but the underlying mechanisms are not fully understood. We investigated the long-term effects of BM-MSCs, particularly in mice with chronic colitis. METHODS: Chronic colitis was induced by administering 3% dextran sulfate sodium (DSS) in a series of three cycles. BMMSCs were injected intravenously into DSS-treated mice three times during the first cycle. On day 33, the therapeutic effects were evaluated with clinicopathologic profiles and histological scoring. Inflammatory mediators were measured with real-time polymerase chain reaction. RESULTS: Systemic infusion of BM-MSCs ameliorated the severity of colitis, and body weight restoration was significantly promoted in the BMMSC- treated mice. In addition, BM-MSC treatment showed a sustained beneficial effect throughout the three cycles. Microscopic examination revealed that the mice treated with BM-MSCs had fewer inflammatory infiltrates, a lesser extent of inflammation, and less crypt structure damage compared with mice with DSS-induced colitis. Anti-inflammatory cytokine levels of interleukin-10 were significantly increased in the inflamed colons of BM-MSC-treated mice compared with DSS-induced colitis mice. CONCLUSIONS: Systemic infusion of BM-MSCs at the onset of disease exerted preventive and rapid recovery effects, with long-term immunosuppressive action in mice with repeated DSS-induced chronic colitis.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Won Ho(김원호) ORCID logo https://orcid.org/0000-0002-5682-9972
Kim, Tae Il(김태일) ORCID logo https://orcid.org/0000-0003-4807-890X
Park, Soo Jung(박수정)
Lee, Hyun Jung(이현정)
Cheon, Jae Hee(천재희) ORCID logo https://orcid.org/0000-0002-2282-8904
Hong, Sung Pil(홍성필)
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