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Upregulation of CD47 in Regulatory T Cells in Atopic Dermatitis

 Nara Lee  ;  Jung U Shin  ;  Shan Jin  ;  Ki Na Yun  ;  Jin Young Kim  ;  Chang Ook Park  ;  Seo Hyeong Kim  ;  Ji Yeon Noh  ;  Kwang Hoon Lee 
 YONSEI MEDICAL JOURNAL, Vol.57(6) : 1435-1445, 2016 
Journal Title
Issue Date
Animals ; CD4 Antigens/analysis ; CD47 Antigen/metabolism* ; Dermatitis, Atopic/immunology ; Female ; Humans ; Mice ; Severity of Illness Index ; Spleen/cytology ; Spleen/immunology ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology* ; T-Lymphocytes, Regulatory/cytology ; T-Lymphocytes, Regulatory/immunology ; T-Lymphocytes, Regulatory/metabolism* ; Up-Regulation*
Atopic dermatitis ; CD47 ; Tandem Mass Tags (TMT) ; regulatory T cells
PURPOSE: Regulatory T (Treg) cells are key modulators in the immune system. Recent studies have shown that atopic dermatitis (AD) patients have higher numbers of Treg cells; however, little is known about the specific phenotype and function of Treg cells in AD.

MATERIALS AND METHODS: To identify differentially expressed proteins in peripheral induced Treg cells in AD and naturally derived Treg cells in normal controls, CD4?CD25? Treg cells were isolated from thymus tissue of normal mice and the spleens of AD mice. Membrane proteins were extracted, and quantitative proteomics labeling with Tandem Mass Tags (TMT) was performed, followed by one-dimensional liquid chromatography/tandem mass spectrometry analysis.

RESULTS: Using TMT labeling, we identified 510 proteins, including 63 membrane proteins and 16 plasma membrane proteins. CD47 was one of the upregulated proteins in Treg cells in AD spleens. Although CD47 was expressed in all CD4? and CD8? T cells, a significantly higher expression of CD47 was observed in the Treg cells of AD mice and AD patients than in those of normal mice and healthy controls. Furthermore, Treg cells from the spleen showed a significantly higher expression of CD47 than those from the thymus.

CONCLUSION: We found that CD47 is highly expressed in the Treg cells of AD mice, particularly in the spleen. Based on our results, we propose that CD47(high) Treg cells are likely induced Treg cells and that upregulated CD47 in the Treg cells of AD patients may play a role in the increased population of Treg cells in AD.
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Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Noh, Ji Yeon(노지연)
Park, Chang Ook(박창욱) ORCID logo https://orcid.org/0000-0003-3856-1201
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Lee, Kwang Hoon(이광훈)
Lee, Na Ra(이나라)
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