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HSP70 is associated with the severity of inflammation in chronic rhinosinusitis

Authors
 Min, Hyun Jin  ;  Yoon, Joo-Heon  ;  Kim, Chang-Hoon 
Citation
 AMERICAN JOURNAL OF RHINOLOGY & ALLERGY, Vol.30(4) : 101-106, 2016 
Journal Title
 AMERICAN JOURNAL OF RHINOLOGY & ALLERGY 
ISSN
 1945-8924 
Issue Date
2016
MeSH
Adolescent ; Adult ; Aged ; Cells, Cultured ; Chronic Disease ; Female ; HSP70 Heat-Shock Proteins/analysis ; HSP70 Heat-Shock Proteins/physiology* ; Humans ; Interleukin-8/analysis ; Lipopolysaccharides/pharmacology ; Male ; Middle Aged ; Rhinitis/etiology* ; Rhinitis/surgery ; Severity of Illness Index ; Sinusitis/etiology* ; Sinusitis/surgery ; Teichoic Acids/pharmacology
Keywords
Heat shock protein 70 ; Lund-Mackay score ; chronic rhinosinusitis ; hypoxia ; lipoteichoic acid ; nasal lavage ; pathogenesis
Abstract
BACKGROUND: Nasal secretions include cytokines and inflammatory mediators that are involved in the pathogenesis of upper airway inflammation. OBJECTIVE: We tried to find unknown biomolecules that are involved in the pathogenesis of chronic rhinosinusitis (CRS). METHODS: We collected nasal mucosal secretions from patients who were diagnosed as having CRS and who underwent endoscopic sinus surgery. A total of 63 patients who underwent nasal secretion collection were reviewed. Enzyme-linked immunosorbent assay was performed by using nasal lavage samples to evaluate which biomolecules were associated with the severity of inflammation based on the Lund-Mackay score. By using human nasal epithelial cells, we performed Western blot, real-time polymerase chain reaction, and enzyme-linked immunosorbent assay to evaluate the secretory mechanism of heat shock protein (HSP) 70. RESULTS: We found that the level of interleukin 8 and HSP70 were significantly associated with the Lund-Mackay score and interleukin 17C, C-X-C motif chemokine 10, and HSP27 were not significantly associated. HSP70 was also significantly associated with the surgical outcome of the enrolled patients. Furthermore, we found that exposure to hypoxia and treatment of lipoteichoic acid induced the secretion of HSP70 but that lipopolysaccharide did not induce the secretion of HSP70 in human nasal epithelial cells. CONCLUSION: Our findings indicated that HSP70 might play a role in the pathogenesis of CRS and the possibility of HSP70 as a biomolecule that represents the severity of CRS.
Full Text
http://www.ingentaconnect.com/content/ocean/ajra/2016/00000030/00000004/art00001
DOI
10.2500/ajra.2016.30.4259
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152041
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