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The Anti-inflammatory Effects of Agmatine on Transient Focal Cerebral Ischemia in Diabetic Rats

 Kim, Jeong Min  ;  Lee, Jong Eun  ;  Cheon, So Yeong  ;  Lee, Jae Hoon  ;  Kim, So Yeon  ;  Kam, Eun Hee  ;  Koo, Bon-Nyeo 
 Journal of Neurosurgical Anesthesiology, Vol.28(3) : 203-213, 2016 
Journal Title
 Journal of Neurosurgical Anesthesiology 
Issue Date
Agmatine/pharmacology* ; Animals ; Blotting, Western ; Brain/drug effects* ; Diabetes Mellitus, Experimental ; Disease Models, Animal ; Inflammation/prevention & control* ; Ischemic Attack, Transient/drug therapy* ; Male ; Neuroprotective Agents/pharmacology* ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction
agmatine ; diabetes ; inflammation ; innate immunity ; ischemia-reperfusion injury
BACKGROUND: In the previous study, we observed agmatine (AGM) posttreatment immediately after 30 minutes of suture occlusion of the middle cerebral artery (MCAO) reduced the infarct size and neurological deficit in diabetic rats. The aim of the present study was to investigate the anti-inflammatory effect of AGM to reduce cerebral ischemic damage in diabetic rats. MATERIALS AND METHODS: Normoglycemic (n=20) and streptozotocin-induced diabetic rats (n=40) were subjected to 30 minutes of MCAO followed by reperfusion. Twenty diabetic rats were treated with AGM (100 mg/kg, intraperitoneal) immediately after 30 minutes of MCAO. Modified neurological examinations and rotarod exercises were performed to evaluate motor function. Western blot and immunohistochemical analysis were performed to determine the expression of inflammatory cytokines in ischemic brain tissue. Real-time polymerase chain reaction was performed to measure the mRNA expression of high-mobility group box 1, receptor for advanced glycation end products (RAGE), Toll-like receptor (TLR)2, and TLR4 RESULTS AND CONCLUSIONS:: AGM posttreatment improved the neurobehavioral activity and motor function of diabetic MCAO rats at 24 and 72 hours after reperfusion. Immunohistochemical analysis showed that AGM treatment significantly decreased the expression of inflammatory cytokines in diabetic MCAO rats at 24 and 72 hours after reperfusion (P<0.01). Western blotting and real-time polymerase chain reaction results indicated that AGM treatment significantly decreased the expression of high-mobility group box 1, RAGE, TLR2, and TLR4 in diabetic rats at 24 hours after reperfusion (P<0.05). This neuroprotective effect of AGM after MCAO was associated with modulation of the postischemic neuronal inflammation cascade.
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1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
구본녀(Koo, Bon-Nyeo) ORCID logo https://orcid.org/0000-0002-3189-1673
김소연(Kim, So Yeon) ORCID logo https://orcid.org/0000-0001-5352-157X
김정민(Kim, Jeongmin) ORCID logo https://orcid.org/0000-0002-0468-8012
이재훈(Lee, Jae Hoon) ORCID logo https://orcid.org/0000-0001-6679-2782
이종은(Lee, Jong Eun) ORCID logo https://orcid.org/0000-0001-6203-7413
천소영(Cheon, So Yeong)
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