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In Vivo Application of Bacteriophage as a Potential Therapeutic Agent To Control OXA-66-Like Carbapenemase-Producing Acinetobacter baumannii Strains Belonging to Sequence Type 357

Authors
 Jongsoo Jeon  ;  Choong-Min Ryu  ;  Jun-Young Lee  ;  Jong-Hwan Park  ;  Dongeun Yong  ;  Kyungwon Lee 
Citation
 Applied and Environmental Microbiology, Vol.82(14) : 4200-4208, 2016 
Journal Title
 Applied and Environmental Microbiology 
ISSN
 0099-2240 
Issue Date
2016
MeSH
Acinetobacter Infections/therapy* ; Acinetobacter baumannii/classification ; Acinetobacter baumannii/enzymology* ; Acinetobacter baumannii/growth & development ; Acinetobacter baumannii/virology* ; Animals ; Bacterial Proteins/secretion* ; Bacteriophages/growth & development* ; Bacteriophages/isolation & purification ; Bacteriophages/physiology ; Bacteriophages/ultrastructure ; Computational Biology ; Disease Models, Animal ; Genome, Viral ; Genotype ; Host Specificity ; Hydrogen-Ion Concentration ; Lung/microbiology ; Lung/pathology ; Mice ; Microscopy, Electron, Transmission ; Multilocus Sequence Typing ; Phage Therapy/methods* ; Pneumonia, Bacterial/therapy* ; Sequence Analysis, DNA ; Survival Analysis ; Time Factors ; Treatment Outcome ; Virion/ultrastructure ; Virus Attachment ; beta-Lactamases/secretion*
Abstract
The increasing prevalence of carbapenem-resistant Acinetobacter baumannii (CRAB) strains in intensive care units has caused major problems in public health worldwide. Our aim was to determine whether this phage could be used as an alternative therapeutic agent against multidrug-resistant bacterial strains, specifically CRAB clinical isolates, using a mouse model. Ten bacteriophages that caused lysis in CRAB strains, including blaOXA-66-like genes, were isolated. YMC13/01/C62 ABA BP (phage B?-C62), which showed the strongest lysis activity, was chosen for further study by transmission electron microscopy (TEM), host range test, one-step growth and phage adsorption rate, thermal and pH stability, bacteriolytic activity test, genome sequencing and bioinformatics analysis, and therapeutic effect of phage using a mouse intranasal infection model. The phage B?-C62 displayed high stability at various temperatures and pH values and strong cell lysis activity in vitro The phage B?-C62 genome has a double-stranded linear DNA with a length of 44,844 bp, and known virulence genes were not identified in silico. In vivo study showed that all mice treated with phage B?-C62 survived after intranasal bacterial challenge. Bacterial clearance in the lung was observed within 3 days after bacterial challenge, and histologic damage also improved significantly; moreover, no side effects were observed. IMPORTANCE: In our study, the novel A. baumannii phage B?-C62 was characterized and evaluated in vitro, in silico, and in vivo These results, including strong lytic activities and the improvement of survival rates, showed the therapeutic potential of the phage B?-C62 as an antimicrobial agent. This study reports the potential of a novel phage as a therapeutic candidate or nontoxic disinfectant against CRAB clinical isolates in vitro and in vivo.
Files in This Item:
T201602980.pdf Download
DOI
10.1128/AEM.00526-16
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
용동은(Yong, Dong Eun) ORCID logo https://orcid.org/0000-0002-1225-8477
이경원(Lee, Kyungwon) ORCID logo https://orcid.org/0000-0003-3788-2134
전종수(Jeon, Jong Soo) ORCID logo https://orcid.org/0000-0002-7213-5663
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151871
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