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In Vivo Application of Bacteriophage as a Potential Therapeutic Agent To Control OXA-66-Like Carbapenemase-Producing Acinetobacter baumannii Strains Belonging to Sequence Type 357

DC FieldValueLanguage
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author전종수-
dc.date.accessioned2017-10-26T07:18:54Z-
dc.date.available2017-10-26T07:18:54Z-
dc.date.issued2016-
dc.identifier.issn0099-2240-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151871-
dc.description.abstractThe increasing prevalence of carbapenem-resistant Acinetobacter baumannii (CRAB) strains in intensive care units has caused major problems in public health worldwide. Our aim was to determine whether this phage could be used as an alternative therapeutic agent against multidrug-resistant bacterial strains, specifically CRAB clinical isolates, using a mouse model. Ten bacteriophages that caused lysis in CRAB strains, including blaOXA-66-like genes, were isolated. YMC13/01/C62 ABA BP (phage B?-C62), which showed the strongest lysis activity, was chosen for further study by transmission electron microscopy (TEM), host range test, one-step growth and phage adsorption rate, thermal and pH stability, bacteriolytic activity test, genome sequencing and bioinformatics analysis, and therapeutic effect of phage using a mouse intranasal infection model. The phage B?-C62 displayed high stability at various temperatures and pH values and strong cell lysis activity in vitro The phage B?-C62 genome has a double-stranded linear DNA with a length of 44,844 bp, and known virulence genes were not identified in silico. In vivo study showed that all mice treated with phage B?-C62 survived after intranasal bacterial challenge. Bacterial clearance in the lung was observed within 3 days after bacterial challenge, and histologic damage also improved significantly; moreover, no side effects were observed. IMPORTANCE: In our study, the novel A. baumannii phage B?-C62 was characterized and evaluated in vitro, in silico, and in vivo These results, including strong lytic activities and the improvement of survival rates, showed the therapeutic potential of the phage B?-C62 as an antimicrobial agent. This study reports the potential of a novel phage as a therapeutic candidate or nontoxic disinfectant against CRAB clinical isolates in vitro and in vivo.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.publisherAmerican Society for Microbiology-
dc.relation.isPartOfAPPLIED AND ENVIRONMENTAL MICROBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcinetobacter Infections/therapy*-
dc.subject.MESHAcinetobacter baumannii/classification-
dc.subject.MESHAcinetobacter baumannii/enzymology*-
dc.subject.MESHAcinetobacter baumannii/growth & development-
dc.subject.MESHAcinetobacter baumannii/virology*-
dc.subject.MESHAnimals-
dc.subject.MESHBacterial Proteins/secretion*-
dc.subject.MESHBacteriophages/growth & development*-
dc.subject.MESHBacteriophages/isolation & purification-
dc.subject.MESHBacteriophages/physiology-
dc.subject.MESHBacteriophages/ultrastructure-
dc.subject.MESHComputational Biology-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHGenome, Viral-
dc.subject.MESHGenotype-
dc.subject.MESHHost Specificity-
dc.subject.MESHHydrogen-Ion Concentration-
dc.subject.MESHLung/microbiology-
dc.subject.MESHLung/pathology-
dc.subject.MESHMice-
dc.subject.MESHMicroscopy, Electron, Transmission-
dc.subject.MESHMultilocus Sequence Typing-
dc.subject.MESHPhage Therapy/methods*-
dc.subject.MESHPneumonia, Bacterial/therapy*-
dc.subject.MESHSequence Analysis, DNA-
dc.subject.MESHSurvival Analysis-
dc.subject.MESHTime Factors-
dc.subject.MESHTreatment Outcome-
dc.subject.MESHVirion/ultrastructure-
dc.subject.MESHVirus Attachment-
dc.subject.MESHbeta-Lactamases/secretion*-
dc.titleIn Vivo Application of Bacteriophage as a Potential Therapeutic Agent To Control OXA-66-Like Carbapenemase-Producing Acinetobacter baumannii Strains Belonging to Sequence Type 357-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Laboratory Medicine-
dc.contributor.googleauthorJongsoo Jeon-
dc.contributor.googleauthorChoong-Min Ryu-
dc.contributor.googleauthorJun-Young Lee-
dc.contributor.googleauthorJong-Hwan Park-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorKyungwon Lee-
dc.identifier.doi10.1128/AEM.00526-16-
dc.contributor.localIdA02649-
dc.contributor.localIdA02423-
dc.relation.journalcodeJ00197-
dc.identifier.eissn1098-5336-
dc.identifier.pmid27208124-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.citation.volume82-
dc.citation.number14-
dc.citation.startPage4200-
dc.citation.endPage4208-
dc.identifier.bibliographicCitationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol.82(14) : 4200-4208, 2016-
dc.date.modified2017-10-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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