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Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells

Authors
 Yun-Min Kook  ;  Young Mi Kang  ;  Seong Hwan Moon  ;  Won-Gun Koh 
Citation
 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, Vol.38 : 113-122, 2016 
Journal Title
 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY 
ISSN
 1226-086X 
Issue Date
2016
Keywords
Co-culture ; Intervertebral disk cells ; Mesenchymal stem cells ; Bi-compartmental scaffold
Abstract
The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmental hydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogel domain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the bare alginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiber-incorporated hydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluorescence staining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups produced more collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than the single-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It is expected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for the study of various cell?cell interactions
Full Text
http://www.sciencedirect.com/science/article/pii/S1226086X1630079X
DOI
10.1016/j.jiec.2016.04.013
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Young Mi(강영미) ORCID logo https://orcid.org/0000-0002-7686-6316
Moon, Seong Hwan(문성환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151707
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