Cited 14 times in
Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 문성환 | - |
dc.contributor.author | 강영미 | - |
dc.date.accessioned | 2017-10-26T07:11:09Z | - |
dc.date.available | 2017-10-26T07:11:09Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1226-086X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151707 | - |
dc.description.abstract | The combination of electrospinning and the subsequent gelation of alginate produced bi-compartmental hydrogel consisting of a nanofiber-incorporated hydrogel matrix domain and a bare alginate hydrogel domain. The co-culture system was prepared by placing intervertebral disk (IVD) cells in the bare alginate hydrogel and human bone marrow mesenchymal stem cells (hMSCs) in the nanofiber-incorporated hydrogel. Real-time polymerase chain reaction (PCR), western blotting, immunofluorescence staining, and sulfated glycosaminoglycan assays revealed that the co-cultured groups produced more collagen type II, aggrecan, glucose transporter-1 (GLUT-1), and glycosaminoglycans (GAG) than the single-cultured hMSCs, confirming the enhanced differentiation of hMSCs in the co-culture system. It is expected that our bi-compartmental 3D scaffold can be applied to heterotypic co-culture systems for the study of various cell?cell interactions | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Bi-compartmental 3D scaffolds for the co-culture of intervertebral disk cells and mesenchymal stem cells | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Orthopedic Surgery | - |
dc.contributor.googleauthor | Yun-Min Kook | - |
dc.contributor.googleauthor | Young Mi Kang | - |
dc.contributor.googleauthor | Seong Hwan Moon | - |
dc.contributor.googleauthor | Won-Gun Koh | - |
dc.identifier.doi | 10.1016/j.jiec.2016.04.013 | - |
dc.contributor.localId | A01365 | - |
dc.relation.journalcode | J03016 | - |
dc.relation.journalsince | 1995 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1226086X1630079X | - |
dc.subject.keyword | Co-culture | - |
dc.subject.keyword | Intervertebral disk cells | - |
dc.subject.keyword | Mesenchymal stem cells | - |
dc.subject.keyword | Bi-compartmental scaffold | - |
dc.contributor.alternativeName | Moon, Seong Hwan | - |
dc.contributor.affiliatedAuthor | Moon, Seong Hwan | - |
dc.citation.volume | 38 | - |
dc.citation.startPage | 113 | - |
dc.citation.endPage | 122 | - |
dc.identifier.bibliographicCitation | JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, Vol.38 : 113-122, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 45721 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.