0 235

Cited 9 times in

Identification of ganglioside GM2 activator playing a role in cancer cell migration through proteomic analysis of breast cancer secretomes

Authors
 Jihye Shin  ;  Gamin Kim  ;  Jong Won Lee  ;  Ji Eun Lee  ;  Yoo Seok Kim  ;  Jong?Han Yu  ;  Seung?Taek Lee  ;  Sei Hyun Ahn  ;  Hoguen Kim  ;  Cheolju Lee 
Citation
 CANCER SCIENCE, Vol.107(6) : 828-835, 2016 
Journal Title
 CANCER SCIENCE 
ISSN
 1347-9032 
Issue Date
2016
MeSH
Biomarkers, Tumor/analysis ; Biomarkers, Tumor/blood ; Biomarkers, Tumor/metabolism ; Blotting, Western ; Breast Neoplasms/blood ; Breast Neoplasms/pathology* ; Breast Neoplasms/secretion* ; Case-Control Studies ; Cell Line, Tumor ; Cell Movement*/genetics ; Disease Progression ; Enzyme-Linked Immunosorbent Assay ; Female ; G(M2) Activator Protein/deficiency ; G(M2) Activator Protein/metabolism* ; Humans ; Neoplasm Proteins/analysis ; Neoplasm Proteins/blood ; Neoplasm Proteins/metabolism ; Neoplasm Staging ; Proteome/analysis ; Proteome/secretion* ; Proteomics*
Keywords
Biomarker ; breast cancer ; ganglioside GM2 activator ; migration ; secretome
Abstract
Cancer cell secretomes are considered a potential source for the discovery of cancer markers. In this study, the secretomes of four breast cancer (BC) cell lines (Hs578T, MCF-7, MDA-MB-231, and SK-BR-3) were profiled with liquid chromatography-tandem mass spectrometry analysis. A total of 1410 proteins were identified with less than 1% false discovery rate, of which approximately 55% (796 proteins) were predicted to be secreted from cells. To find BC-specific proteins among the secreted proteins, data of immunohistochemical staining compiled in the Human Protein Atlas were investigated by comparing the data of BC tissues with those of normal tissues. By applying various criteria, including higher expression level in BC tissues, higher predicted potential of secretion, and sufficient number of tandem mass spectra, 12 biomarker candidate proteins including ganglioside GM2 activator (GM2A) were selected for confirmation. Western blot analysis and ELISA for plasma samples of healthy controls and BC patients revealed elevation of GM2A in BC patients, especially those who were estrogen receptor-negative. Additionally, siRNA-mediated knockdown of GM2A in BC cells decreased migration in vitro, whereas the overexpression of GM2A led to an increase in cell migration. Although GM2A as a diagnostic and prognostic marker in BC should be carefully verified further, this study has established the potential role of GM2A in BC progression.
DOI
10.1111/cas.12935
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Gamin(김가민) ORCID logo https://orcid.org/0000-0001-8504-7921
Kim, Hogeun(김호근)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151635
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links