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Identification of ganglioside GM2 activator playing a role in cancer cell migration through proteomic analysis of breast cancer secretomes

DC FieldValueLanguage
dc.contributor.author김호근-
dc.contributor.author김가민-
dc.date.accessioned2017-10-26T07:07:40Z-
dc.date.available2017-10-26T07:07:40Z-
dc.date.issued2016-
dc.identifier.issn1347-9032-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151635-
dc.description.abstractCancer cell secretomes are considered a potential source for the discovery of cancer markers. In this study, the secretomes of four breast cancer (BC) cell lines (Hs578T, MCF-7, MDA-MB-231, and SK-BR-3) were profiled with liquid chromatography-tandem mass spectrometry analysis. A total of 1410 proteins were identified with less than 1% false discovery rate, of which approximately 55% (796 proteins) were predicted to be secreted from cells. To find BC-specific proteins among the secreted proteins, data of immunohistochemical staining compiled in the Human Protein Atlas were investigated by comparing the data of BC tissues with those of normal tissues. By applying various criteria, including higher expression level in BC tissues, higher predicted potential of secretion, and sufficient number of tandem mass spectra, 12 biomarker candidate proteins including ganglioside GM2 activator (GM2A) were selected for confirmation. Western blot analysis and ELISA for plasma samples of healthy controls and BC patients revealed elevation of GM2A in BC patients, especially those who were estrogen receptor-negative. Additionally, siRNA-mediated knockdown of GM2A in BC cells decreased migration in vitro, whereas the overexpression of GM2A led to an increase in cell migration. Although GM2A as a diagnostic and prognostic marker in BC should be carefully verified further, this study has established the potential role of GM2A in BC progression.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherWiley Publishing on behalf of the Japanese Cancer Association-
dc.relation.isPartOfCANCER SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBiomarkers, Tumor/analysis-
dc.subject.MESHBiomarkers, Tumor/blood-
dc.subject.MESHBiomarkers, Tumor/metabolism-
dc.subject.MESHBlotting, Western-
dc.subject.MESHBreast Neoplasms/blood-
dc.subject.MESHBreast Neoplasms/pathology*-
dc.subject.MESHBreast Neoplasms/secretion*-
dc.subject.MESHCase-Control Studies-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Movement*/genetics-
dc.subject.MESHDisease Progression-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHFemale-
dc.subject.MESHG(M2) Activator Protein/deficiency-
dc.subject.MESHG(M2) Activator Protein/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHNeoplasm Proteins/analysis-
dc.subject.MESHNeoplasm Proteins/blood-
dc.subject.MESHNeoplasm Proteins/metabolism-
dc.subject.MESHNeoplasm Staging-
dc.subject.MESHProteome/analysis-
dc.subject.MESHProteome/secretion*-
dc.subject.MESHProteomics*-
dc.titleIdentification of ganglioside GM2 activator playing a role in cancer cell migration through proteomic analysis of breast cancer secretomes-
dc.typeArticle-
dc.publisher.locationEngland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Pathology-
dc.contributor.googleauthorJihye Shin-
dc.contributor.googleauthorGamin Kim-
dc.contributor.googleauthorJong Won Lee-
dc.contributor.googleauthorJi Eun Lee-
dc.contributor.googleauthorYoo Seok Kim-
dc.contributor.googleauthorJong?Han Yu-
dc.contributor.googleauthorSeung?Taek Lee-
dc.contributor.googleauthorSei Hyun Ahn-
dc.contributor.googleauthorHoguen Kim-
dc.contributor.googleauthorCheolju Lee-
dc.identifier.doi10.1111/cas.12935-
dc.contributor.localIdA01183-
dc.relation.journalcodeJ00454-
dc.identifier.eissn1349-7006-
dc.relation.journalsince2003~-
dc.identifier.pmid27002480-
dc.relation.journalbefore~2002 Japanese Journal of Cancer Research : Gann-
dc.subject.keywordBiomarker-
dc.subject.keywordbreast cancer-
dc.subject.keywordganglioside GM2 activator-
dc.subject.keywordmigration-
dc.subject.keywordsecretome-
dc.contributor.alternativeNameKim, Ho Keun-
dc.contributor.affiliatedAuthorKim, Ho Keun-
dc.citation.volume107-
dc.citation.number6-
dc.citation.startPage828-
dc.citation.endPage835-
dc.identifier.bibliographicCitationCANCER SCIENCE, Vol.107(6) : 828-835, 2016-
dc.date.modified2017-10-24-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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