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In vivo bone formation by human marrow stromal cells in biodegradable scaffolds that release dexamethasone and ascorbate-2-phosphate

Authors
 Hyongbum Kim  ;  Hwal Suh  ;  Sangmee Ahn Jo  ;  Hyun Woo Kim  ;  Jung Min Lee  ;  Eun Hae Kim  ;  Yvonne Reinwald  ;  Sang-Hyug Park  ;  Byoung-Hyun Min  ;  Inho Jo 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.332(4) : 1053-1060, 2005 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2005
MeSH
Marrow stromal cells ; Scaffolds ; Poly(D,L-lactide-co-glycolide) ; Dexamethasone ; Ascorbate-2-phosphate ; Osteogenesis ; Bone ; Differentiation ; Alkaline phosphatase ; Tissue engineering
Keywords
Marrow stromal cells ; Scaffolds ; Poly(D,L-lactide-co-glycolide) ; Dexamethasone ; Ascorbate-2-phosphate ; Osteogenesis ; Bone ; Differentiation ; Alkaline phosphatase ; Tissue engineering
Abstract
An unsolved problem with stem cell-based engineering of bone tissue is how to provide a microenvironment that promotes the osteogenic differentiation of multipotent stem cells. Previously, we fabricated porous poly(d,l-lactide-co-glycolide) (PLGA) scaffolds that released biologically active dexamethasone (Dex) and ascorbate-2-phosphate (AsP), and that acted as osteogenic scaffolds. To determine whether these osteogenic scaffolds can be used for bone formation in vivo, we seeded multipotent human marrow stromal cells (hMSCs) onto the scaffolds and implanted them subcutaneously into athymic mice. Higher alkaline phosphatase expression was observed in hMSCs in the osteogenic scaffolds compared with that of hMSCs in control scaffolds. Furthermore, there was more calcium deposition and stronger von Kossa staining in the osteogenic scaffolds, which suggested that there was enhanced mineralized bone formation. We failed to detect cartilage in the osteogenic scaffolds (negative Safranin O staining), which implied that there was intramembranous ossification. This is the first study to demonstrate the successful formation of mineralized bone tissue in vivo by hMSCs in PLGA scaffolds that release Dex and AsP.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X05010119
DOI
10.1016/j.bbrc.2005.05.051
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Suh, Hwal(서활)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151369
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