155 338

Cited 0 times in

Interleukin-1β를 처치한 기도상피세포의 프로테옴 및 트랜스크립톰의 분석

Other Titles
 Proteomic and Transcriptomic Analysis of Interleukin-1β Treated Airway Epithelial Cells 
Authors
 김창훈  ;  백승재  ;  김평화  ;  윤주헌 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.48(2) : 158-171, 2005 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
ISSN
 1225-035X 
Issue Date
2005
MeSH
2-Dimensional polyacrylamide gel electrophoresis ; cDNA microarray ; Mucus ; Mucin ; Hypersecretion
Keywords
2-Dimensional polyacrylamide gel electrophoresis ; cDNA microarray ; Mucus ; Mucin ; Hypersecretion
Abstract
Background and Objectives:Mucin hypersecretion is one of the main symptoms of inflammatory diseases in the respiratory tract. The authors previously reported that pleiotypic pro-inflammatory cytokine, interleukin (IL)-1β, plays significant roles in the respiratory tract inflammation by inducing mucins (MUC2, MUC5AC, MUC8). However, the molecular mechanism for mucin hypersecretion in the respiratory tract is still unclear.

Materials and Method:In order to understand the mechanisms of mucin hypersecretion in the airway epithelium, the differentially expressed proteins and genes in the lung mucoepidermoid carcinoma cell line (NCI-H292 cells), which were treated for 6 and 24 hours with IL-1β (10 ng/ml), were identified using 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) proteomics and cDNA microarray analysis (8.6 K).

Results:In the 2-D PAGE, 8 differentially expressed proteins and 14 post-translational modification proteins were identified 6 and 24 hrs after the IL-1β treatment. Microarray analysis identified a total of 413 genes (6.6%) in the 6-hour treatment group and 115 genes (2.0%) in the 24-hour treatment group that were regulated after the IL-1β treatment. The differentially expressed genes that were regulated by the IL-1β treatment were mostly found in the metabolic pathway rather than in the regulatory pathway. A comparison of the proteomic and microarray data showed that there was a large discrepancy between the protein expression and the gene expression levels. Among the genes encoding the proteins secreted in the airway, MUC5B was down-regulated but sialomucin CD 164, lysozyme, and the secretory leukocyte protease inhibitor (SLPI) were up-regulated.

Conclusion:These results clearly show that the transcript levels have little value in predicting the extent of protein expression. Genomics and proteomics have different evaluation fields. Therefore, they may not provide all the information on the gene and protein profiles.
Files in This Item:
T200501067.pdf Download
DOI
OAK-2005-05311
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151207
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links