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정상 사람 코점막 상피세포에서 막특이성 퓨린성 수용체의 발현

Other Titles
 Membrane-Specific Expression of Functional Purinergic Receptors in Normal Human Nasal Epithelial Cells 
Authors
 김창훈  ;  김성식  ;  유종범  ;  이세영  ;  송미현  ;  이정권  ;  윤주헌 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.48(5) : 606-614, 2005 
Journal Title
 Korean Journal of Otolaryngology (대한이비인후과학회지) 
ISSN
 1225-035X 
Issue Date
2005
MeSH
Base Sequence ; Calcium Signaling/physiology ; Cell Membrane/physiology* ; DNA Primers ; Humans ; Kinetics ; Molecular Sequence Data ; Nasal Mucosa/physiology* ; RNA, Messenger/genetics ; Receptors, Purinergic/genetics* ; Reference Values ; Reverse Transcriptase Polymerase Chain Reaction
Keywords
purinoreceptor ; calcium ; secretion
Abstract
Background and Objectives: Extracellular purines and pyrimidines regulate various physiological responses via cell surface receptors known as purinoreceptors, and may exert autocrine or paracrine effects on ion transport, fluid transport, ciliary beat frequency and mucin secretion. This study aims to investigate the expression patterns of such purinoreceptors found in normal human nasal epithelial (NHNE) cells. Materials and Method: In RT-PCR, the mRNAs for several P2X (P2X3, P2X4, P2X7) and P2Y (P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12) receptors were identified in NHNE cells. Functional localizations of P2 receptors were investigated by measuring [Ca2+]i increases in a membrane-specific manner using a double-perfusion chamber. Absence of the responses of -Me ATP and 2MeS-ATP excluded functionally active P2X3, P2X4, and P2Y1 receptors as far as [Ca2+]i increase was concerned. Results: Applications with ATP and UTP revealed that luminal membranes of NHNE cells express P2Y2 and P2Y6 receptors and basolateral membranes P2Y2 receptors. Expressions of P2Y2 and P2Y6 receptors in NHNE cells were further verified by the immunoblotting using specific antibodies. In addition, the results with BzATP indicated that the P2Y11 receptor may be present on the luminal side. Conclusion: The NHNE cells express functionally active P2Y2, P2Y6 and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells.
Files in This Item:
T200501063.pdf Download
DOI
10.1152/ajplung.00285.2003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151199
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