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Retrovirally transduced NCAM140 facilitates neuronal fate choice of hippocampal progenitor cells

Authors
 Ju Hee Kim  ;  Jung-Ha Lee  ;  Jin-Yong Park  ;  Chang-Hwan Park  ;  Chae-Ok Yun  ;  Sang-Hun Lee  ;  Yong-Sung Lee  ;  Hyeon Son 
Citation
 JOURNAL OF NEUROCHEMISTRY, Vol.94(2) : 417-424, 2005 
Journal Title
JOURNAL OF NEUROCHEMISTRY
ISSN
 0022-3042 
Issue Date
2005
MeSH
Animals ; Blotting, Western/methods ; Cell Count/methods ; Cell Differentiation/drug effects ; Cell Differentiation/physiology ; Cells, Cultured ; Drug Interactions ; Embryo, Mammalian ; Enzyme Inhibitors/pharmacology ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Female ; Flavonoids/pharmacology ; Gene Expression/drug effects ; Gene Expression/physiology ; Green Fluorescent Proteins/metabolism ; Hippocampus/cytology* ; Hippocampus/embryology ; Immunohistochemistry/methods ; Indoles ; Microtubule-Associated Proteins/metabolism ; Neural Cell Adhesion Molecule L1/metabolism ; Neural Cell Adhesion Molecules/physiology* ; Neurons/metabolism* ; Neurons/virology ; Pregnancy ; Protein Isoforms/metabolism ; Proto-Oncogene Proteins c-bcl-2/metabolism ; RNA, Messenger/metabolism ; Rats ; Retroviridae/genetics* ; Retroviridae/physiology ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Sialic Acids/metabolism ; Stem Cells/drug effects ; Stem Cells/physiology* ; Stem Cells/virology ; Transduction, Genetic/methods* ; Tubulin/metabolism
Keywords
extracellular signal‐regulated kinase ; hippocampus ; neural cell adhesion molecule ; neurogenesis ; rat
Abstract
Neural cell adhesion molecule (NCAM) influences proliferation and differentiation of neuronal cells. However, only a little is known about the downstream effects of NCAM signalling, such as alterations in gene transcription, which are associated with cell fate choice. To examine whether NCAM plays a role in cell fate choice during hippocampal neurogenesis, we performed a gain-of-function study, using a retroviral vector which contained full-length NCAM140 cDNA and the marker gene EGFP, and found that NCAM140 promoted neurogenesis by activating proneural transcription activators with concurrent inhibition of gliogenesis. The enhanced transcript levels of proneural transcription factors in NCAM140-transduced cells were down-regulated by treatment of the cells with mitogen-activated protein kinase kinase (MEK) inhibitor PD098059. Overall, these findings suggest that NCAM140 may facilitate hippocampal neurogenesis via regulation of proneurogenic transcription factors in an extracellular signal-regulated kinase (ERK)-dependent manner.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2005.03208.x/abstract
DOI
10.1111/j.1471-4159.2005.03208.x
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
Yonsei Authors
Yun, Chae Ok(윤채옥)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151029
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